- 中文名称
Erk1, Phosphorylated (T202/Y204), Erk2, Phosphorylated (T185/Y187) Plate BioAssay ELISA Kit
- 英文名字
- Erk1, Phosphorylated (T202/Y204), Erk2, Phosphorylated (T185/Y187) Plate BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- E3452-50C
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:For the development of sandwich ELISAs to measure ERK1 phosphorylated at T202/Y204 and ERK2 phosphorylated at T185/Y187 in cell lysates.Test Principle:This BioAssay ELISA Kit contains the basic components required for the development of sandwich ELISAs to measure ERK1 dually phosphorylated at T202/Y204 and ERK2 dually phosphorylated at T185/Y187 in cell lysates. Immobilized capture antibodies specific for ERK1 and ERK2 bind both phosphorylated and unphosphorylated protein. After washing away unbound material, a biotinylated detection antibody specific for ERK1 or ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively, is used to detect only phosphorylated protein, utilizing a standard Streptavidin-HRP format.Specificity:This IC ELISA specifically recognizes ERK1 dually phosphorylated at T202 and Y204, and ERK2 dually phosphorylated at T185 and Y187. Specificity was demonstrated by Western blot analysis of the protein bound by the capture antibody supplied in the kit, cross-reactivity analysis, and peptide competition.Cross-reactivity experiments were performed with this IC ELISA to further determine specificity. Unphosphorylated recombinant human (rh) JNK1 and rhp38a were assayed at 300ng/ml and did not cross-react or interfere in the assay. Unphosphorylated rhERK1 and rhERK2 were assayed at 300ng/ml and measured 2280pg/ml (0.78% cross-reactivity) and 3150pg/ml (1.15 % cross-reactivity), respectively.Kit Components: Contains sufficient materials to run ELISAs on at least two 96 well plates.Phospho-ERK1/ERK2 Capture Antibody: 1440mg/ml x vial when reconstituted with 200ml of PBS.Phospho-ERK1/ERK2 Detection Antibody: 14.4mg/ml x vial when reconstituted with 1ml of IC diluentPhospho-ERK1/ERK2 Standard: 1230ng/ml x vial when reconstituted with 500ml of IC diluentStreptavidin (HRP): 1x1mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Erk1, Phosphorylated (T202/Y204), Erk2, Phosphorylated (T185/Y187) Plate BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Erk1, Phosphorylated (T202/Y204), Erk2, Phosphorylated (T185/Y187) Plate BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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