- 中文名称
Epstein-Barr Virus-Viral Capsid Antigen (EBV-VCA) IgM, Human, BioAssay ELISA Kit
- 英文名字
- Epstein-Barr Virus-Viral Capsid Antigen (EBV-VCA) IgM, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- E3440-14M
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The United States Biological Epstein-Barr Virus-Viral Capsid Antigen (EBV-VCA) IgM, Human, ELISA Kit is intended for the detection of IgM antibody to Epstein-Barr virus in human serum.Detection of the Epstein-Barr virus was first described in 1964 by Epstein, Achong, and Barr using electron microscopic studies of cultured lymphoblasts derived from patients with Burkitt’s lymphoma1. EBV is classified as a member of the herpes-virus family based upon its characteristic morphology (2,3.) EBV infection may demonstrate a wide spectrum of clinical symptoms. The majority of primary EBV infections are transmitted via saliva, occur during childhood, and are subclinical (4). In the U.S., 50% of the population demonstrate EBV antibodies before the age of 5 years; 80% by adulthood. Transfusion- associated EBV infections have also been reported (3). In young adults, EBV infection may be clinically manifested as Infectious Mononucleosis (IM) with typical symptoms of sore throat, fever, and lymphadenopathy (3). College students and military personnel are often cited as a high morbidity incidence population for IM (3). Following primary EBV infection, it is postulated that the B lymphocyte may continue to harbor the EBV genome and establish a latent infection that may extend through life (4). Reactivation of EBV infection or enhanced EBV activation has been documented in immunodeficient or immunosuppressed patients, i.e., organ transplant patients, individuals with malignancies, pregnant women, and persons of advanced age (4). Epstein-Barr virus has also been associated in the pathogenesis of two sample cancers, Burkitt’s lymphoma and nasopharyngeal carcinoma. Documentation by means of DNA hybridization studies demonstrates the presence of the EBV genome on biopsy specimens taken from individuals with these carcinomas (3). Burkitt’s lymphoma is primarily observed in Sub-Sahara Africa, especially in African children, and in New Guinea. Malarial infections are usually diagnosed in Burkitt’s lymphoma samples and are suggested to be a co-factor (5,6). Nasopharyngeal carcinoma is observed in Asia, most notably in Southern China, and may have genetic or environmental influences as the co-factors (5,6). In the last two decades, serological methods have progressed from testing for the presence of non-specific heterophile antibodies to measuring levels of IgG or IgM formed against subunits of EBV antigen complexes. One of the best indicators of active EBV infection is antibody to viral capsid antigens, structural proteins necessary for replication of the virus (7). Viral capsid antigens are present in every cell infected with EBV. The IgM response to VCA is among the earliest detectable humoral immune responses, usually present at the onset of the disease and peaking within four to six weeks. VCA-IgM levels are also transient, declining rapidly and usually becoming undetectable within two to three months from onset of clinical symptoms (8).Principle of the Assay:Purified EBV-VCA antigen is coated on the surface of microwells. Diluted sample serum is added to wells, and the EBV-VCA IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. Kit Components:T8171-92D1: Microwell strips, 12x8 wells. EBV-VCA antigen coated wells. T8171-92D2: Absorbent solution, 1x22ml. Black CapT8171-92D3: Washing concentrate (10X), 1x100ml. T8171-92D4: TMB Chromogenic Substrate, 1x15ml. Amber bottle.T8171-92D5: Enzyme conjugate, 1x12ml. Red color solutionT8171-92D6: Negative control, 1x150ul. Range stated on label. Natural CapT8171-92D7: Cut-off calibrator, 1x150ul. Yellow CapT8171-92D8: Positive control, 1x150ul. Range stated on label. Red Cap. T8171-92D9: Stop solution, 1x12ml. 2 N HCl Storage and Stability:Unopened kit is stable for at least 6 months at 4°C. Once opened, use up all wells within 4 weeks after initial opening of the pouch. Always keep microwells tightly sealed in pouch with desiccants. Do not expose test reagents to heat, sun, or strong light during storage or usage.
- 产品特点
- 针对Epstein-Barr Virus-Viral Capsid Antigen (EBV-VCA) IgM, Human, BioAssay ELISA Kit 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Epstein-Barr Virus-Viral Capsid Antigen (EBV-VCA) IgM, Human, BioAssay ELISA Kit 产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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