- 中文名称
Epidermal Growth Factor, Human (EGF) BioAssay ELISA Kit
- 英文名字
- Epidermal Growth Factor, Human (EGF) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- E3374-15H
- 产品报价
- ￥23
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Human Epidermal Growth Factor (Hu EGF) ELISA is to be used for the in vitro quantitative determination of human EGF in human serum, plasma, buffered solution, cell culture medium or urine. The assay will recognize both natural and recombinant human EGF.Epidermal Growth Factor (EGF) is a 53-amino acid polypeptide originally discovered in the mouse submaxillary gland as an agent that induced precocious eyelid opening and early incisor eruption in the newborn mouse. EGF has been subsequently shown to elicit an array of biological responses that are mediated by specific binding to an EGF receptor/tyrosine kinase located on the cell surface. EGF induces cell proliferation, regulates tissue differentiation, modulates organogenesis, promotes angiogenesis, and accelerates wound healing. It also acts as an inhibitor of gastric acid secretion. EGF is a member of a family of EGF-related growth factors including TGFa, heparin binding EGF-like growth factor (HB-EGF), epiregulin, amphiregulin (AR), betracellulin (BTC), neuregulin 1, neuregulin 2, and neuregulin 3. EGF is initially synthesized as a precursor of a 160-170kD glycoprotein. The mature and soluble form of EGF is a ∼6kD protein which can be detected in many tissues and nearly all body fluids. High levels of EGF exist in some body fluids such as the saliva, urine, gastric juice, and mammary and seminal fluids. EGF, triggers an intracellular signaling cascade through binding to EGF receptor (also known as ErbB1), causing changes within the target cells.The EGF receptor, a receptor tyrosine kinase, upon stimulation by EGF results in the phosphorylation of its distinct tyrosine residues and phosphorylates the adaptor protein, SHC. Subsequently, the complex activates the Ras/mitogen activated protein (MAP) kinase pathway.This cascade couples EGF stimulation to gene transcription.Sample Type:Serum, Plasma, Urine, Supernatants Intended Use:The Invitrogen Human Epidermal Growth Factor (Hu EGF) ELISA is to be used for the in vitro quantitative determination of Hu EGF in human serum, plasma, buffered solution, cell culture medium or urine. The assay will recognize both natural and recombinant Hu EGF.Sensitivity:<1pg⁄ml Range: 3.9-250pg/mlIncubation Time:4 hours at RTSample Size: 100ulSpecificity:Buffered solutions of a panel of substances at 10,000 pg/ml were assayed with the Invitrogen human EGF kit. The following substances were tested and found to have no cross-reactivity: human IL-1b, IL-2, IL-4, IL-6, IL-10, FGF acidic, FGF basic, G-CSF, GM-CSF, IFN-y, KGF, PDGF-AB, SCF, TGF-a, TGF-b1, TNF-a, TNF-b, VEGF-121, VEGF-165; mouse IL-1b, IL-6, G-CSF, GM-CSF, IFN-γ, TNF-a; rat IL-1a, IL-6, GM-CSF, IFN-y, TNF-a.Test Principle:The human EGF kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A polyclonal antibody specific for human EGF has been coated onto the wells of the microtiterstrips provided. Samples, including standards of known human EGF content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the Hu EGF antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for Hu EGF is added. During the second incubation, this antibody binds to the immobilized Hu EGF captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing toremove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu EGF present in the original specimen.Accuracy: Inter-assay CV: ≤5.9%Intra-assay CV: ≤5.0%Kit Components:*E3374-15H1: Hu EGF Standard, purified recombinant Hu EGF expressed in E. coli. Refer to vial label for quantity and reconstitution volume. 2vials, 4 vialsE3374-15H2: Standard Diluent Buffer 15mM sodium azide 1x25ml, 2x25mlE3374-15H3: Plate Covers, adhesive strips. E3374-15H4: Hu EGF Antibody-Coated Wells, 96 wells per plate. 1plate, 2platesHu EGF Biotin Conjugate (Biotin-labeled anti-EGF). Contains 15 mM sodium azide; 1x11ml, 2x11mlE3374-15H5: Streptavidin-Peroxidase (HRP), (100x) concentrate. Contains 3.3 mM thymol; 1x0.125ml, 2x125mlE3374-15H6: Streptavidin-Peroxidase (HRP) Diluent. Contains 3.3mM thymol; 1x25mlE3374-15H7: Wash Buffer Concentrate (25x); 1x100mlE3374-15H8: Stabilized Chromogen, Tetramethylbenzidine (TMB) 1x25ml E3374-15H9: Stop Solution 1x25mlStorage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Epidermal Growth Factor, Human (EGF) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Epidermal Growth Factor, Human (EGF) BioAssay ELISA Kit产品后将其置于4°C Do not freeze保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
