- 中文名称
Elk-1 (Ser383) BioAssay Phosphorylation Detection Kit discontinued
- 英文名字
- Elk-1 (Ser383) BioAssay Phosphorylation Detection Kit discontinued
- 供应商
- USBiological
- 产品货号
- E2240-40
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Synthetic phospho-Ser383 peptide (KLH coupled) corresponding to residues around Ser383 of human Elk-1.The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1–3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus and phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1 (Figure 1). Elk-1 appears to be a direct target of activated MAP kinase, as biochemical studies indicate that Elk-1 is a good substrate for MAP kinase, the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk-1 activation in vivo. More recent studies have shown that Elk-1 (Ser383) is also a target of the Stress activated kinase SAPK/JNK (4,5). The Elk-1 (Ser383) Antibody Kit provides reagents and protocols for the rapid analysis of Elk-1 phosphorylation status. The Kit includes a phospho-antibody that recognizes serine 383 phosphorylated Elk-1, an Elk-1 antibody (phosphorylation-state independent), protein controls and protein markers for western blots and the HRP Western Detection System.Applications: Suitable for use in Western Blotting. Other applications not tested.Recommended Dilution:Western blotting: 1:1000Both phospho-and control Elk-1 antibodies are useful in determining Elkphosphorylation status by western blotting (Figure 2). The low levels of endogenous Elk-1 protein present in most cell types make it difficult to detect by direct western blotting.Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- 产品特点
- 针对Elk-1 (Ser383) BioAssay Phosphorylation Detection Kit discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Elk-1 (Ser383) BioAssay Phosphorylation Detection Kit discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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