- 中文名称
DNA Polymerase, Tsg, Recombinant (Tsg), BioGenomics Kit
- 英文名字
- DNA Polymerase, Tsg, Recombinant (Tsg), BioGenomics Kit
- 供应商
- USBiological
- 产品货号
- D3947
- 产品报价
- ￥23
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Tsg DNA Polymerase is a thermostable DNA Polymerase isolated from a strain of Thermus sp. Tsg has a half life of 3 hours at 95°C and is very stable. Tsg has high fidelity with an error frequency 10/10e6 (or 0.01/10e3) during DNA synthesis. Tsg is designed for use in primer extension reaction. Tsg (Tsg) can also be used for sequencing. DNA sequencing at high temperature may decrease the secondary structure of some DNA templates and permit polymerization through base-paired region. DNA sequencing with Tsg DNA Polymerase produces uniform band intensities and low background.Unit Definition:One unit incorporates 10nmole of dNTP into acid-insoluble material in 30min. at 74°C.Kit Components:1. Recombinant Tsg DNA Polymerase: 5 units/ul in 100mM KCl, 20mM Tris HCl (pH 8.0, 22°C), 0.1mM EDTA, 0.5mM PMSF, 1mM DTT, 50% glycerol.2. 10X Taq Reaction Buffer (Mg+2 free): (Included as D3947-01) Supplied as a liquid in 100mM potassium chloride, 100mM (NH4)2SO4, 200mM Tris HCl, pH 8.75 at RT, 1% Triton X-100, 1mg/ml BSA. Buffer is optimized for use with 200uM dNTPs.3. Magnesium Sulfate (20mM MgSO4): (Included as D3947-02)Supplied as a liquid in 20mM MgSO4. The final magnesium sulfate may be varied according to individual requirements. In general, 2mM MgSO4 is recommended.Primer Extension Characteristics:Tsg has the independent terminal transferase activity which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product. TA cloning vector is recommended if the extension product is needed to be cloned. This product has not been licensed for PCR use.Performance & Quality Testing:Tsg DNA Polymerase is highly purified free of contaminating endonucleases, exonucleases and nicking activity. The purity of the D3947 is also evaluated by adding 10units of Tsg DNA Polymerase in 100ul of a reaction mixture for making first strand cDNA at the beginning and no impaired effect on the first strand is observed.Endonuclease Assay: 1ug of Lambda HindIII DNA is incubated with 20 units of the D3947 in assay buffer at 75°C for 16 hrs. No visible contaminating activity is observed.Exonuclease Assay: 1ug of pBR322 plasmid DNA is incubated with 10 units of the D3947 in assay buffer at 75°C for 16 hrs. No detectable exonuclease is observed.Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对DNA Polymerase, Tsg, Recombinant (Tsg), BioGenomics Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到DNA Polymerase, Tsg, Recombinant (Tsg), BioGenomics Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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