- 中文名称
DNA Assay Kit, BioAssay
- 英文名字
- DNA Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- D3878-07
- 产品报价
- ￥1/250Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling.Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at 450nm (lexc=350nm), is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.Key Features:Sensitive and accurate. Linear detection range 2-40ng (100–2000ng/mL) calf thymus DNA in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.Applications:Direct Assays: plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.Kit Contents: (250 tests in 96-well plates)Reagent: 50ml Standard: 1ml 10 ug/mL calf thymus DNAStorage conditions. The kit is shipped at room temperature. Store the DNA standard at -20°C and the Reagent at 2-8°C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Preparation: bring reagents to room temperature before use.Procedure using 96-well plate:1. Prepare 400ul 2000 ng/mL Premix by mixing 80ul Standard and 320ul TE buffer (10mM Tris, 1mM EDTA, pH 7.4). Dilute standards as follows. Transfer 20ul diluted standards and samples into wells of a black flat-bottom 96-well plate. Store standards at 4°C for future use.No Premix+TE Vol (ul) DNA (ng/mL)1 100ul+0ul 100 20002 80ul+20ul 100 16003 60ul+40ul 100 12004 40ul+60ul 100 8005 30ul+70ul 100 6006 20ul+80ul 100 4007 10ul+90ul 100 2008 0ul+100ul 100 02. Add 200ul working reagent and tap lightly to mix.3. Incubate 1 min at room temperature and read fluorescence emission at 440-460nm (peak 450nm, excitation at 340-370 nm).Procedure using cuvette:1. Transfer 100ul diluted standards and samples to cuvets.2. Add 1000ul working reagent and tap lightly to mix.3. Incubate 1 min at room temperature and read fluorescence intensity at 440-460nm (peak 450nm, excitation at 340-360 nm).Calculation:Subtract blank fluorescence value (water, #8) from the standard values and plot DF against standard DNA concentrations. Determine the slope using linear regression fitting. The DNA concentration of Sample is calculated as=FSAMPLE–FBLANKSlope X n (ng/mL)FSAMPLE and FBLANK are fluorescence values of the sample and water or buffer in which the sample was diluted, respectively. n is the sample dilution factor.Materials Required, But Not Provided:Pipeting devices and accessories. 1 x TE buffer.Procedure using 96-well plate:Black 96-well plates (e.g. Corning) and fluorescence plate reader.Procedure using cuvette:Fluorescence spectrophotometer and fluorometric cuvets.General Considerations:(1) For samples with unknown DNA concentration, pipet 1ul sample and mix with 99ul TE buffer. Make further serial 10-fold dilutions. Assay all diluted samples and choose dilutions at which the fluorescence intensity values fall within the linear calibration range to calculate sample DNA concentration. (2). Calf thymus DNA serves as a standard for plant and animal DNA, because the AT content is conserved among most DNAs from these two species. For bacterial DNA, a different standard should be used that best matches the sample DNA content. (3). Fluorescence intensity is half when binding to the same single-stranded DNA. Short single-stranded DNA pieces do not fluoresce with this dye.
- 产品特点
- 针对DNA Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到DNA Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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