- 中文名称
DNA Isolation Kit, Blood and Serum, BioAssay
- 英文名字
- DNA Isolation Kit, Blood and Serum, BioAssay
- 供应商
- USBiological
- 产品货号
- D3878-03C
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Blood and Serum DNA Isolation Kit is designed for the purification of not only genomic DNA from whole blood or cells, but also genomic DNA fragment (circulating DNA) from serum, plasma, and bio fluid in a spin column format. No phenol-chloroform extraction, no Protease, and no precipitation steps are involved. The sample addition and washing steps can be performed using compatible vacuum manifold, while the lysis of cells and the final elution of the DNA product are performed using a table-top centrifuge.The blood cells in whole blood or other cultured cells and serum are first lysed in the lysis binding buffer that contains the denaturant guanidine HCl and Triton X100. Ethanol is then added to the samples, which are then added to the filter spin columns. This step facilitates the binding of DNA to the filter matrix. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.Quality Control:A representative kit from the same lot is randomly selected for blood genomic DNA isolation from 200ul fresh whole blood. The performance should be as follows:Yield:3ug-9ugPurity:UV260/280> 1.7DNA Size:99% of DNA is > 20kbTotal time of prep less than 25 minItemAmountStorage1. Lysis Binding Buffer21mlRT2. Wash Buffer 126mlRT3. Wash Buffer 212mlRT4. TE10mlRT5. spin column set100 unitsRT
- 产品特点
- 针对DNA Isolation Kit, Blood and Serum, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到DNA Isolation Kit, Blood and Serum, BioAssay产品后将其置于RT保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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