Anti-Host Cell Protein (HCP) Antibody Production, Option A - CUST92C-Anti-Host Cell Protein (HCP) Antibody Production, Option A

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中文名称: Anti-Host Cell Protein (HCP) Antibody Production, Option A - CUST92C

英文名字: Anti-Host Cell Protein (HCP) Antibody Production, Option A - CUST92C

供应商: Rockland

产品货号: CUST92C

产品报价: ￥询价/1Each

产品说明书: 点击查看

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产品新闻

背景资料: Complete service includes immunizing the HCP preparation after fractionation into high molecular weight (HMW) and low molecular weight (LMW) fractions by ultra filtration (provided by the client). Typically a membrane with a nominal molecular weight cutoff of 30,000 daltons is used to separate proteins less than 30kDa from those greater than 30 kDa. Separately HMW and LMW proteins are immunized into New Zealand White female rabbits. Six rabbits are immunized per immunogen. Project includes: 2 X 6 RABBIT hosts, 90 days daily maintenance, 12 Pre-bleeds (up to 5 mL each), 24 Test bleeds (up to 5 mL each), 20 production bleeds (4 production bleeds from the 5 best responding RABBITS) of up to 25 mL each or up to 500 mL). After a series of immunizations and test bleeds, reactivity against HCPs is assessed by western blot. Only host animals showing reactivity to a broad range of proteins by size are selected for production. Terminal bleeds (1 each from the best responding 5 RABBITS of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client. After selection, host animal production bleeds are pooled and then purified by a method equivalent to Protein A chromatography (7S fractionation or IgY purification from eggs) up to 1.0 L of qualified antiserum. Antibodies to HMW HCP proteins and antibodies to LMW HCP man be combined after purification and validation at the client's request. Vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysates. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.

产品描述: Complete service includes separation of the host cell protein into high molecular weight (HMW) and low molecular weight (LMW) fractions by ultra filtration, 10 RABBIT host animals, 90 days RABBIT daily maintenance, 10 RABBIT Pre-bleeds (5 mL each), 10 RABBIT test bleeds (5 mL each), 20 RABBIT production bleeds (4 production bleeds from the 5 best responding animals of 25 mL each or up to 500 mL), 5 Rabbit terminal bleeds (1 each from the 5 best responding animals of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client, Protein A purification of up to 1.0 L of qualified antiserum, vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysate. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.

产品形式: 该Anti-Host Cell Protein (HCP) Antibody Production, Option A - CUST92C的详细信息查看Rockland提供的产品说明书

保存建议: 该Anti-Host Cell Protein (HCP) Antibody Production, Option A - CUST92C的详细信息查看Rockland提供的产品说明书

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。