中文名称

TFP-dPEG® β-生物素酶抗性生物素-1000 mg

英文名字

TFP-dPEG®₄-biotinidase resistant biotin-1000 mg

供应商

Click Chemistry Tools

产品货号

CCT-QBD-10203-1000

产品报价

￥询价/1000mg

产品说明书

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购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系。本公司所有产品仅供科研使用，不用于临床诊断。

产品新闻

背景资料

TFP-dPEG®4-生物素酶抗性生物素，产品编号QBD-10203，是一种经修饰以抵抗生物素酶水解的水溶性生物素化试剂(EC 3.5.1.12)。该产品将生物素酰基-2-氨基丁酸与单分子量离散聚乙二醇(dPEG®)连接体的2，3，5，6-四氟苯基(TFP)酯结合。TFP-dPEG®4-生物素酶抗性生物素保留了生物素对抗生物素蛋白和链霉抗生物素蛋白的典型高亲和力，但也阻断了生物素酶对生物素的作用。该产品可用于组织样品的ELISA和抗体预靶向等应用，其中内源性生物素酶可回收生物素。

产品描述

TFP-dPEG®4-biotinidase-resistant biotin, product number QBD-10203, is a water-soluble biotinylation reagent modified to resist hydrolysis by biotinidase (EC 3.5.1.12). This product combines Biotinoyl-2-aminobutyric acid with the 2,3,5,6-tetrafluorophenyl (TFP) ester of a single molecular weight, discrete polyethylene glycol (dPEG®) linker. TFP-dPEG®4-biotinidase-resistant biotin retains biotin’s typical high affinity for avidin and streptavidin but also blocks the action of biotinidase on biotin. This product is useful in applications such as ELISAs of tissue samples and antibody pretargeting, where endogenous biotinidase can recycle biotin.nBiotinidase is an amidohydrolase that breaks the covalent bond between biotin and amino acids such as lysine (i.e., biocytin). In vivo, biotinidase recycles biotin. Although necessary for healthy bodily function, biotinidase potentially creates problems when biotinylated proteins are used in serological assays, since endogenous biotinidase removes the biotin from these proteins.nDr. D. Scott Wilbur and colleagues at the University of Washington in Seattle developed biotinidase-resistant biotin in 2006 to facilitate in vivo antibody pretargeting. However, to improve the product’s water solubility, a short amino-dPEG®4-propionic acid linker was conjugated to the biotinidase-resistant biotin and subsequently functionalized as an TFP ester. This product is essentially the same as QBD-10202, but the TFP ester is more stable to hydrolysis in aqueous media and has a higher optimal pH range of 7.5 - 8.5 compared to NHS esters.nLike our other dPEG® biotinylation products, TFP-dPEG®4-biotinidase-resistant biotin, QBD-10203, will not cause aggregation and precipitation of biomolecules to which it is conjugated, and the dPEG® spacer reduces non-specific binding.nTFP-dPEG®4-biotinidase-resistant biotin resists amide hydrolysis because of the non-natural amino acid to which it is conjugated. It is potentially beneficial for applications using serological fluids that contain endogenous biotinidase. Useful roles for TFP-dPEG®4-biotinidase-resistant biotin include the following:ntherapeutic applications such as antibody pretargeting with biotin-streptavidin systems;nsupramolecular constructs (e.g., nanoparticles) used in vivo that depend on biotin-(strept)avidin affinity to maintain structural integrity; and,nELISAs of serological fluids and tissue samples using biotin-(strept)avidin affinity.

产品形式

白色至类白色固体

保存建议

其他

Click Chemistry Tools（CCT）公司位于美国亚利桑那州斯科茨代尔（Scottsdale,AZ）。自公司成立以来，一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外，CCT还可以提供个性化的定制产品，已满足不同用户的需求。目前CCT的产品线主要包括：Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。nFluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，自成立以来，一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外，Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。nQuanta Biodesign公司位于美国中部的俄亥俄州，成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合，生产出他们独有的专利产品dPEG Building Blocks and Linkers，被广泛用于细胞治疗和分子诊断等研究领域。另外，还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。n目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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