Western blotting: 0.5-5ug/ml;Immunocytochemistry in formalin fixed cells: 5-30ug/ml;Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml;Immunohistochemistry in paraffin section: 5-30ug/ml;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml;Optimal working dilutions must be determined by end user.