- 中文名称
CREB (Ser133) BioAssay Phosphorylation Detection Kit discontinued
- 英文名字
- CREB (Ser133) BioAssay Phosphorylation Detection Kit discontinued
- 供应商
- USBiological
- 产品货号
- C7915-14
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including cAMP, membrane depolarization, increased intracellular Ca++, and growth and neurotrophic factors (1–3) (Figure 1). Phosphorylation of CREB at Ser133 regulates the ability of CREB to activate transcription when bound at a CRE. Mutation of Ser133 renders CREB nonresponsive to multiple signaling pathways. A variety of protein kinases have been shown to phosphorylate CREB at Ser133 in vitro, including PKA, PKC, CAM kinase II and IV, and p90rsk. Phosphorylation at Ser133 has also been shown to promote interaction with a CREB binding protein, CBP, required for transcriptional activation by CREB, AP-1, and SRF dependent promoters (3,4). CREB appears to play an important role in learning and memory in both flies and mice. Mice lacking CREB exhibit deficiencies in spatial learning tasks, while flies overexpressing and lacking CREB show enhanced or diminished learning (5,6). The CREB (Ser133) Antibody Kit provides reagents and protocols for the rapid analysis of CREB (Ser133) phosphorylation status. The Kit includes a phospho-antibody that recognizes CREB (Ser133) only when phosphorylated at Ser133, a control CREB antibody (phosphorylation-state independent), protein controls and protein markers for western blots, and the HRPApplications: Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.Kit Components:CREB (48H2) Rabbit Mab (43kD)1x100 ul Phospho-CREB (Ser133)Mab Rb (43kD) 1x100 ul CREB Control Cell Extracts 1x40ulIgG (HRP) goat x rabbit, 1x50ul Biotin (HRP) goat x Rabbit 1x100ul 20X LumiGLO® Reagent and 20X Peroxide 1x5mlBiotinylated Protein Ladder 1x100ulRecommended Dilution:Western blotting: 1:1000Immunoprecipitation: 1:250 Immunocytochemistry: 1:1000 Immunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- 产品特点
- 针对CREB (Ser133) BioAssay Phosphorylation Detection Kit discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到CREB (Ser133) BioAssay Phosphorylation Detection Kit discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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