- 中文名称
Creatinine Assay Kit, BioAssay
- 英文名字
- Creatinine Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- C7911-48
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Creatinine is synthesized in the body at a fairly constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and hence the urine levels are diminished. Creatinine test is most widely used to assess kidney function.Simple, direct and automation-ready procedures for measuring creatinine concentration in biological samples are becoming popular in Research and Drug Discovery. Creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.Key Features:Sensitive and accurate. Use 30ul samples. Detection limit 0.10 mg/dL (8uM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples.Applications:Direct Assays: urine, serum, plasma and biological preparations.Drug Discovery/Pharmacology: effects of drugs on creatinine metabolism.Kit Contents: (500 tests in 96-well plates) Reagent A: 50mlReagent B: 50ml Creatinine Standard: 1ml 50mg/dLStorage conditions. The kit is shipped at room temperature. Store all components at 2-8°C. For long-term storage, keep standard at –20°C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Equilibrate reagents to room temperature prior to use. Please note the difference in standard/sample volume and Working Reagent strength for blood and urine assays. This assay is based on a kinetic Jaffe reaction. To ensure identical incubation time, addition of Working Reagent to standard and samples should be quick and mixing should be brief but thorough. Use of a multi-channel pipettor is recommended.Procedure using 96-well plate:Blood Assay (Low Creatinine Linear Up To 50 mg/dL):1. Dilute standard to 2 mg/dL by mixing 5ul 50 mg/dL standard stock and 120ul distilled water. Transfer 30ul diluted standard and serum/plasma in duplicate into wells of a clear bottom 96-well plate.2. Prepare enough Working Reagent by mixing per well reaction at least 100ul Reagent A and 100ul Reagent B. Add 200ul Working Reagent quickly to all wells. Tap plate briefly to mix.3. Read optical density immediately (OD0) and then at 5 min (OD5) at 490-530nm (peak absorbance at 510nm).Urine Assay (High Creatinine Linear Up To 300 mg/dL):1. Transfer 5ul 50 mg/dL standard and urine in duplicate into wells of a clear bottom 96-well plate.2. Prepare enough Working Reagent by mixing per well reaction 50ul Reagent A, 50ul Reagent B and 100ul water. Add 200ul Working Reagent quickly to all wells. Tap plate briefly to mix.3. Read optical density immediately (OD0) and then at 5 min (OD5) at 490-530nm (peak absorbance at 510nm).Procedure using cuvette:1. Transfer 100ul 2 mg/dL Standard and serum/plasma samples (Urine Assay: 15ul 50 mg/dL Standard and 15ul urine) to cuvets.2. Prepare appropriate Working Reagent as above for the 96-well plate Procedures:. Add 1000ul Working Reagent to each cuvet and pipet briefly to mix (avoid bubble formation).3. Read OD immediately (OD0) and at 5 min (OD5) at 490-530nm.Calculation:Creatinine concentration of the sample is calculated as =ODSAMPLE 5–ODSAMPLE0ODSTD 5–ODSTD 0x [STD] (mg/dL)ODSAMPLE5, ODSAMPLE0, ODSTD5 and ODSTD0 are OD510nm values of sample and standard at 5 and 0 min, respectively. [STD] is 2 mg/dL for blood assay and 50 mg/dL for urine assay.Conversions: 1mg/dL creatinine equals 88.4uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices and accessories (e.g. multi-channel pipettor). Clear bottom 96-well plates (e.g. Corning Costar) and plate reader for the plate procedure. Spectrophotometer and cuvets for measuring OD 510nm for the cuvette procedure.Examples:Samples were assayed in duplicate (n=2) using the 96-well plate protocol. The creatinine concentration (mg/dL) was 0.38 ± 0.01 for rat serum, 0.71 ± 0.02 for rat plasma, 0.79 ± 0.00 for human serum, 0.89 ± 0.04 for human plasma, 1.20 ± 0.04 for goat serum and 136.4 ± 2.9 in a fresh human urine sample.
- 产品特点
- 针对Creatinine Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Creatinine Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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