- 中文名称
Creatine Kinase Assay Kit, BioAssay
- 英文名字
- Creatine Kinase Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- C7909-97
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Creatine Kinase (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis. Simple, direct and automation-ready procedures for measuring CK activity are very desirable. Creatine Kinase Assay Kit is based on enzyme coupled reactions in which creatine phosphate and ADP is converted to creatine and ATP by CK, the generated ATP is used to phosphorylate glucose by hexokinase to generate glucose-6-phosphate, which is then oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase. The produced NADPH, measured at 340 nm, is proportionate to the CK activity in the sample.Applications:Direct Assays: CK in serum, plasma and other biological samples.Pharmacology: effects of drugs on CK activity.Key Features:Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Kit Contents: (100 tests in 96-well plates)Assay Buffer: 12ml Substrate Solution: 1.0mlEnzyme Mix: 120ul Calibrator: 150ulStorage conditions. This kit is shipped on dry ice. Store all reagents at -20°C. Shelf life: 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Sample Preparation. Use non-hemolyzed samples. Samples should be assayed within 4 hours of blood collection if they remain at room temperature, or within 12 hours if stored at 4°C. Samples can be stored at -80°C and should be thawed only once. If turbidity is observed, centrifuge sample and use clear supernatant for assay. Reagent Reconstitution. Bring all components to room temperature. For each reaction well, mix 10ul Substrate Solution, 100ul Assay Buffer and 1ul Enzyme Mix. Fresh reconstitution is recommended. If the assay is to be carried out at 37°C, warm up the Reconstituted Reagent at 37°C.1. Calibrator: transfer 110ul water and (10ul Calibrator+100ul water) into separate wells of a clear bottom 96-well plate.Samples: transfer 10ul samples into separate wells. Add 100ul Reconstituted Reagent and tap plate to mix.2. Reaction. Incubate at room temperature or 37°C. CK is fully activated within 20 min by glutathione provided in the Substrate Solution. Read OD340nm at 20 min and again at 40 min.3. Calculation:. Calculate sample CK activity using the equation,CK (U/L) =OD40min–OD20minODCALIBRATOR–ODH2O× 150OD40min and OD20min are OD340nm values at 40 min and 20 min for the sample. ODCALIBRATOR and ODH2O are OD340nm values of the Calibrator and water blank at 40 min. The value 150 is the equivalent activity (U/L) of the Calibrator under the assay conditions. Unit definition: one unit of CK will transfer 1uMole of phosphate from phosphocreatine to ADP per min at pH 6.0. Note: If the CK activity is expected to be higher than 300 U/L, read OD340nm at 20 min and again at 25 min. To calculate the CK activity replace (OD40min–OD20min) with (OD25min–OD20min) and replace the factor 150 with 600 in the above equation. Linear range: 30 to 1,800U/L CK activity.Materials Required, But Not Provided:Pipeting (multi-channel) devices. Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.General Considerations:This assay is based on an enzyme-catalyzed kinetic reaction. Addition of Reconstituted Reagent should be quick and mixing should be brief but thorough. Use of multi-channel pipettor is recommended.Examples:Samples were assayed in duplicate using the 96-well protocol. The CK activity (U/L) was 12.0 ± 0.9 for a rat serum sample, 11.0 ± 0.5 for human serum, 28 ± 1 for human plasma, 9.0 ± 0.8 for mouse serum and 49 ± 2 for bovine serum.
- 产品特点
- 针对Creatine Kinase Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Creatine Kinase Assay Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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