Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development. The Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (lambda-max = 570nm), and highly fluorescent (Ex/Em = 538/587nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10mM Creatine.Kit Components:Creatine Assay Buffer 1x25mlCreatine Probe lyophilizedDMSO (Anhydrous) 1x0.4mlCreatinase lyophilizedCreatine Enzyme mix lyophilizedCreatine Standard (10umol) lyophilized.Storage and Stability:Store the kit at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
