- 中文名称
Cortisol, Human, BioAssay ELISA Kit
- 英文名字
- Cortisol, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- C7904-20B
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The United States Biological Cortisol, Human, BioAssay ELISA Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol concentration in serum and plasma.Cortisol is a steroid hormone released from the adrenal cortex in response to a hormone called ACTH (produced by the pituitary gland). It is involved in the response to stress; it increases blood pressure and blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. In plasma, cortisol is bound to corticosteroid-binding globulin (CBG, transcotin) with high affinity, and to albumin. Only free cortisol is available to most receptors. The amount of cortisol present in the serum undergoes diurnal variation, with the highest levels present in the early morning, and lower levels in the evening, several hours after the onset of sleep. Highest levels are at about 6-8 a.m. and lowest levels are at about midnight. These normal endogenous functions are the basis for the physiological consequences of chronic stress-prolonged cortisol secretion causes muscle wastage, hyperglycemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs. Principle of the Assay:Cortisol (antigen) in the sample competes with enzyme-labeled cortisol HRP-cortisol) for binding onto limited antibody binding sites on the anti-cortisol antibody immobilized on the surface of the microtiter plate. After incubation, bound and free cortisol are separated by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB substrate are then added. After an appropriate amount of time has elapsed for maximum color development, the enzyme reaction is stopped and the absorbance determined. The concentration of cortisol present in the sample is calculated based on a calibration curve constructed from a series of standards. The color intensity is inversely proportional to the cortisol concentration in the sample.Kit Components:Microtiter Plate, 1x96 wells.Cortisol Standard, 0ng/ml, 1x1ml Cortisol Standard, 10ng/ml, 1x1ml Cortisol Standard, 50ng/ml, 1x1ml Cortisol Standard, 150ng/ml, 1x1ml Cortisol Standard, 500ng/ml, 1x1ml HRP-Cortisol, 1x21mlTMB Substrate, 1x12mlStop Solution, 1x12mlStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Cortisol, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Cortisol, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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