- 中文名称
Collagen (Chicken) Type II, Human Antibody (IgA) BioAssay ELISA Kit
- 英文名字
- Collagen (Chicken) Type II, Human Antibody (IgA) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- C7510-35A
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This ELISA kit is designed to assay type II collagen antibodies in human sera with IgA suptype. The microtiter strips provided in the kit have been coated with chicken type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.In general, IgA and IgG antibodies to collagen in human sera are highly specific to a heterologous type I and/or type II collagen regardless of the disease or crossreactivity to human type II collagen. Interestingly, IgA and IgG antibodies in sera from patients react to the same collagen type and species but appear to be inversely proportional to their levels (i.e. if IgG levels are high, IgA levels will be low and vise versa).Reagents Included:This ELISA kit contains enough material to run two partial plates on two separate occasions (see assay procedure). 1. Type II or type I collagen-coated 8 well stripsa) Five, 8-well strips which are uncoated to serve as a control.b) Five, 8-well strips coated with either CII, BII, PII and HII (Human Type II ELISA) or coated with either CI, BI, PI and HI (Human Type I ELISA).c) Four, 8-well strips to be used for reference standards. Two strips are used per run allowing two partial plates to be run two separate occasions.2. Standard antibody (1.1ml, 16units/ml).3. Solution A (Blocking buffer, 15ml)4. Solution B (Sample dilution buffer, 60ml): Use for diluting the standard and sample specimens.5. Secondary antibody (two vials of biotin-conjugated goat anti-human IgA, lyophilized): Dissolve one vial in 10ml Solution C-1.6. Solution C-1 (Secondary antibody dilution buffer, 20ml).7. Peroxidase-conjugated Streptavidin (two vials)Dissolve one vial in 10ml Solution C-2.8. Solution C-2 (Avidin-Peroxidase dilution buffer, 20ml)9. Urea-H2O2 plus buffer tablet (two tablets). Dissolve one tablet in 20 ml glass-distilled water.10. OPD tablet (two tablets): Dissolve one tablet in 20ml Urea-H2O2 buffer prepared in step 9, just before use.11. Solution D (Stop solution: 7.5ml of 2.5N Sulfuric acid).12. Solution E (20X Wash buffer, 50ml): Dilute in 1 liter of distilled water.
- 产品特点
- 针对Collagen (Chicken) Type II, Human Antibody (IgA) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Collagen (Chicken) Type II, Human Antibody (IgA) BioAssay ELISA Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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