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中文名称

Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit

英文名字
Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit
供应商
USBiological
产品货号
C7510-34C
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
This ELISA kit is designed to assay type II collagen antibodies in rat sera. The microtiter strips provided in the kit have been coated with rat type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.Heterologous type II collagen is widely used for inducing arthritis in susceptible animals. The serum antibodies in collagen-induced arthritic (CIA) mice are highly specific to the type II collagen used for immunization, however there is significant crossreactivity with other species of type II collagen including rat type II collagen.Kit Components:1. Type II collagen-coated 8-well stripsa) Ten, 8 well strips coated with one species of type II collagen for assaying antibody levels in sample specimens. b) Four, 8 well strips for reference standards. Two strips are used per run allowing two partial plates to be run on two separate occasions.2. Standard antibody (1.2ml, 16units/ml)3. Solution A (Blocking buffer, 15ml)4. Solution B (Sample dilution buffer, 60ml): Use for diluting standards and sample specimens.5. Secondary antibody (two vials of lyophilized, peroxidase-conjugated goat anti-rat IgG): Dissolve one vial in 10ml of solution C (Secondary antibody dilution buffer, 20ml)6. Urea-H2O2 plus buffer tablet containing (two tablets): Dissolve one tablet in 20ml glass-distilled water.7. OPD tablet (two tablets): Dissolve one tablet in 20ml Urea-H2O2 buffer (see step 6 above), just before use.8. Solution D Stop solution: (2.5N Sulfuric acid, 7.5ml)9. Solution E 20X Wash buffer (50ml): dilute in 1 liter of distilled water.
产品特点
针对Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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