- 中文名称
CNM Compartmental Protein Extraction Kit, BioAssay
- 英文名字
- CNM Compartmental Protein Extraction Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- C6012
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Components in this kit are prepared with pure chemicals according to the proprietary technology. To prevent protein degradation, a ready-to-use protease inhibitor cocktail is provided. One Kit is consisted of five reagents enough to enrich four compartment proteins from 5 grams tissues or about 125 million cells. The efficiency of subcellular fractionation has been investigated by SDS-PAGE and immunoblotting of selected marker proteins. Comparing with CNMCS kit, CNM kit has everything except CS buffer for cytoskeleton protein extraction.Quality Control:One kit of this lot has been tested to go through the complete compartmental protein extraction procedure from rat colon. Four compartmental proteins from the extraction are used for electrophoresis, transferring to PVDF membrane and immunoblotting with mouse anti-GAPDH, anti- Histone H1, anti-Vimentin, and Na/K+ ATPase as primary antibodies, and HRP-conjugated anti- Mouse IgG as secondary antibody. The separation of four compartment proteins is confirmed by the enrichment of GAPDH in the cytoplasmic fraction, Histone H1 in the nuclear fraction, Na/K+ ATPase in the membrane fraction, and the unique localization of Vimentin in the cytoskeleton fraction.Reagents are sufficient for extraction of compartment proteins from 5g tissue or about 1.25x10e6 cells. Minimal 0.1g tissue or 2.5x10e6 cells are required.Kit Components:C6012A: Buffer C: HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium orthoVanadate, 1x12mlC6012B: Buffer M: HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40, sodium orthoVanadate, 1x6mlC6012C: Buffer N: HEPES, pH 7.9, MgCl2, sodium chloride, EDTA, glycerol, sodium orthoVanadate, 1x6mlC6012D: Buffer W: HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium orthoVanadate, 1x50mlC6012E: Protease Inhibitors (50X): A cocktail of protease inhibitors, 1x500ulStorage and Stability:Store *C6012E at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对CNM Compartmental Protein Extraction Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到CNM Compartmental Protein Extraction Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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