- 中文名称
Clara Cell Protein, BioAssay ELISA Kit (Club Cell Protein, CC16, CC10, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein)
- 英文名字
- Clara Cell Protein, BioAssay ELISA Kit (Club Cell Protein, CC16, CC10, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein)
- 供应商
- USBiological
- 产品货号
- C5828-02
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human Clara Cell Protein ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Clara Cell Protein serum and plasma (EDTA, citrate, heparin).Human Clara Cell Protein (CC16, CC10 and also called uteroglobin, urinary protein 1 or Clara Cell Secretory Protein) belongs to the family of secretoglobins. It is a secreted protein product of non-ciliated bronchiolar Clara cells. Its function remains to be elucidated. There is convincing data suggesting its phospholipase A2 inhibitory activity as well as a number of other immunomodulatory features including inhibition of interferon gamma signaling and Th1 vs. Th2 lymphocyte regulation. It was proposed as a potential peripheral marker of respiratory epithelial injury and bronchial dysfunction. Clara Cell Protein 16 concentrations have been determined in both serum and bronchoalveolar lavage fluid in numerous studies since 1994. In serum, its increase is associated with age, asbestos, nitrogen chloride and ozone exposure, sarcoidosis and high PEEP ventilation. Decreased serum CC16 levels are found after pulmonary resection, in silica-exposed workers, smokers and in asthma. Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetopathy, Turner and Down syndrome). In pleural effusions, the CC16 concentration appears to be associated with its diffusion from the lung as evidenced by high CC16 levels in cardiac pleural congestion.Features: • The total assay time is less than four hours.• The kit measures homodimeric Clara Cell Protein.• Quality Controls are human serum based. No animal sera are used.• Suitable for use with human serum or plasma samplesTest Principle: In the Human Clara Cell Protein ELISA, calibrators or samples are incubated with a polyclonal rabbit anti-human Clara Cell Protein antibody coated in microtiter wells. After one-hour incubation and a washing, polyclonal anti-human Clara Cell Protein antibody labeled with biotin is added and incubated with captured Clara Cell Protein. After a thorough wash, streptavidin labeled with HRP is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2- tetramethylbenzidine. The reaction is stopped by addition of acidic solution. Absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of Clara Cell Protein. A standard curve is constructed by plotting absorbance values versus Clara Cell Protein concentrations of calibrators. Concentrations of unknown samples are determined using this standard curve.Performance Characteristics:Sensitivity: 46pg/ml; defined as such a concentration of Clara Cell Protein in which the absorbance is higher than the value of three standard deviations of the absorbance of blank added to mean absorbance of blank3xSD(blank) + A(blank)Specificity: Human Clara Cell Protein Range:1.57-50ng/ml Clara Cell Protein/ml of sampleSample Volume:100ul/wellAssay Time:Total assay time is 3-4 hours.Kit Components:C5828-02A: Microtiter Strips, coated with capture polyclonal antibody, sealed, 1x96 wells*C5828-02B: Clara Cell Protein Master Calibrator, 2x1vial*C5828-02C: Quality Controls: High, 11.6-21.6ng/ml, 2x1vial*C5828-02D: Quality Controls: Low, 3.40-6.31ng/ml, 2x1vialC5828-02E: Pab (Biotin), Ready to use, 1x13mlC5828-02F: Streptavidin (HRP), Ready to use, 1x13mlC5828-02G: Dilution Buffer, Ready to use, 1x20mlC5828-02H: Wash Solution, 10X, 1x100mlC5828-02J: TMB Substrate Solution, Ready to use, 1x13mlC5828-02K: Stop Solution, Ready to use, 1x13mlStorage and Stability: Store at 4°C. Stable for 6 months after receipt at 4°C. Store *C5828-02B, *C5828-02C and *C5828-02D at 4°C as a lyophilized powder. Upon reconstitution, aliquot and store the undiluted standard and controls at -20°C. Stable for 3 months.
- 产品特点
- 针对Clara Cell Protein, BioAssay ELISA Kit (Club Cell Protein, CC16, CC10, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Clara Cell Protein, BioAssay ELISA Kit (Club Cell Protein, CC16, CC10, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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