- 中文名称
c-Jun, Total, Sandwich ELISA Kit (Qualitative) discontinued
- 英文名字
- c-Jun, Total, Sandwich ELISA Kit (Qualitative) discontinued
- 供应商
- USBiological
- 产品货号
- C5815-10H
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Total c-Jun Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay that detects endogenous levels of total c-Jun protein. A c-Jun rabbit monoclonal antibody has been coated onto the microwells. After incubation with cell lysates, c-Jun protein is captured by the coated antibody. Following extensive washing, c-Jun mouse monoclonal antibody is added to detect the captured c-Jun protein. HRP-linked anti-mouse antibody is then used to recognize the bound detection antibody antibody. HRP substrate, TMB, is added to develop color color. The magnitude of optical density for this developed color is proportional to the quantity of total c-Jun protein. Antibodies in this kit are custom formulations specific to the kit.c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component ofthe transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (Reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun vival has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13).Specificity/Sensitivity:This kit detects endogenous levels of total c-Jun protein. As shown in Figure 1, using a Phospho-c-Jun (Ser63) Sandwich ELISA kit, a significant induction of phospho-c-Jun (Ser63) in UV- treated 293 cells can be detected. However, the level of total c-Jun protein (phospho and non-phospho), detected by the Total c-Jun Sandwich ELISA kit remains unchanged. Both C6 and NIH/3T3 cells treated with UV light or anisomycin show similar results (data not shown).Kit Components:C5815-10H1: Microtiter Plate, 1x96 wells (12 strips of 8 wells). Coated with rabbit monoclonal c-Jun antibodyC5815-10H2: c-Jun Detection Antibody, 1x11ml. Green solution.C5815-10H3: IgG-HRP, 1x11ml. Anti-mouse IgG linked to HRP. Red solution.C5815-10H4: TMB Substrate, 1x11ml. Colorless solution.C5815-10H5: Stop Solution, 1x11ml. Colorless solutionC5815-10H6: Wash Buffer (20X), 1x25ml. Colorless solution.C5815-10H7: Sample Diluent, 1x25ml. Blue solution.C5815-10H8: Cell Lysis Buffer (10X), 1x15ml. Yellowish solution.Storage and Stability:Store kit at 4°C, with the exception of the 10X Cell Lysis Buffer which needs to be stored at -20°C (packaged separately). Kit is stable for up to 6 months.
- 产品特点
- 针对c-Jun, Total, Sandwich ELISA Kit (Qualitative) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到c-Jun, Total, Sandwich ELISA Kit (Qualitative) discontinued产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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