- 中文名称
Chorionic Gonadotropin, Human (hCG) BioAssay ELISA Kit
- 英文名字
- Chorionic Gonadotropin, Human (hCG) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- C5069-08V
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human chorionic gonadotropin (hCG) is a glycoprotein hormone normally produced by the placenta during pregnancy. The hCG molecule consists of two combined, dissimilar subunits designated alpha and beta. The beta subunit, with a molecular weight of approximately 30,000D, confers biological and immunological specificity to the entire hCG molecule by virtue of its unique amino acid sequence and content. The alpha subunit, with a molecular weight of approximately 18,000D, is essentially identical to the alpha subunit of the pituitary glycoprotein hormones: luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroidstimulating hormone (TSH). The appearance of hCG in urine or serum soon after conception and its rapid rise in concentration makes it an ideal indicator for the detection and confirmation of pregnancy. However, elevated hCG levels are also frequently associated with trophoblastic and nontrophoblastic neoplasmas; these conditions should be consideredbefore a diagnosis of pregnancy can be made. Immunoassays utilizing antibodies specific to the beta subunit of hCG provide a sensitive and specific technique allowing early detection of pregnancy around the time of the first missed menstrual period. In women with a multiple pregnancy (twins, triplets, etc.), levels of hCG have been reported to be higher than those expected during a normal single pregnancy. This is probably the result of the increased placental mass necessary to sustain multiple fetuses. Also, as one might suspect, cases of placental insufficiency show levels of hCG lower than those expected during normal pregnancy. Decreased values have also been associated with threatened abortion and ectopic pregnancy.Sample Type:SerumIntended Use:For the quantitative determination of Human Chorionic Gonadotropin (hCG) concentration in human serum.Test Principle:The hCG Quantitative Test is based on a solid phase enzymelinked immunosorbent assays (ELISA). The assay system utilizes a mouse monoclonal anti-α-hCG antibody for solid phase (microtiter wells) immobilization and a mouse monoclonal anti-b-hCG antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test specimen (serum) is added to the α-hCG antibody coated microtiter wells and incubated with the Zero Bufferat room temperature for 30 minutes. If hCG is present in the specimen, it will combine with the antibody on the wells. The wells are then washed to remove any residual test specimen, and b-hCG monoclonal antibody labeled with horseradish peroxidase (conjugate) is added. The conjugate will bind immunologically to the hCG on the wells, resulting in the hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation at room temperature for 15 minutes, the wells are washed with water to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated at room temperature for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of hCG is directly proportional to the color intensity of the test sample.Kit Components:Mouse monoclonal anti a-hCG coated microtiter plate, 1x96 wells.Zero Buffer, 1x13ml.Enzyme Conjugate Reagent, 1x18ml.Standard, 0mIU/ml, 1x1vialStandard, 5mIU/ml, 1x1vialStandard, 20mIU/ml, 1x1vialStandard, 50mIU/ml, 1x1vialStandard, 150mIU/ml, 1x1vialStandard, 300mIU/ml, 1x1vial TMB Reagent, 1x11ml.Stop Solution (1N HCl), 1x11ml.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Chorionic Gonadotropin, Human (hCG) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Chorionic Gonadotropin, Human (hCG) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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