- 中文名称
Cholesterol Assay Kit, BioAssay, Colorimetric
- 英文名字
- Cholesterol Assay Kit, BioAssay, Colorimetric
- 供应商
- USBiological
- 产品货号
- C5045-10
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. Cholesterol Assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene- 3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.Applications:Direct Assays: cholesterol in serum, plasma, and other biological samples.Pharmacology: effects of drugs on cholesterol metabolism.Key Features:Sensitive and accurate. Detection limit of 5mg/dL, linearity up to 300mg/dL cholesterol in 96-well plate assay.Convenient. Room temperature assay. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Kit Contents: (100 tests in 96-well plates)Assay Buffer: 20ml Enzyme Mix: 120uLNAD Solution: 2 x 1ml Standard: 1ml 300mg/dL cholesterolStorage conditions. Store reagents at -20°C. Shelf life: 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Important: bring all reagents to room temperature prior to assay. Serum and plasma samples should be clear and free of turbidity or precipitates. If present, precipitates should be removed by filtration or centrifugation in a table centrifuge. If not assayed immediately, samples can be stored at -20 to -80°C for at least one year.1. Standard Curve. Prepare a 10-fold diluted standard (STD) by mixing 40ul 300mg/dL Standard and 360ul Assay Buffer. Further dilute standard (STD) in Assay Buffer as shown below.No STD+Assay Buffer Vol (ul) 10 x Conc. (mg/dL)1 100ul+0ul 100 3002 80ul+20ul 100 2403 60ul+40ul 100 1804 40ul+60ul 100 1205 30ul+70ul 100 906 20ul+80ul 100 607 10ul+90ul 100 308 0ul+100ul 100 0Transfer 50ul diluted standards into wells of the 96-well plate. Samples: dilute samples 10-fold (e.g. 10ul sample with 90ul Assay Buffer). Transfer 50ul diluted sample in separate wells.2. Prepare enough NAD solution in Assay Buffer as follows: for each reaction well, mix 40ul Assay Buffer with 18ul the provided NAD Solution. Add 50ul of diluted NAD to standards and sample wells. Tap plate to mix well. Let stand 5 min at room temperature. Read background optical density at 340nm (ODo).3. Prepare enough enzyme mix as follows: for each reaction well, mix 10ul Assay Buffer with 1ul provided Enzyme Mix. Add 10ul diluted enzyme mix per well. Tap plate to mix thoroughly. Note: the enzyme mix may appear to be turbid, but will be clear after mixing into the reaction mixture.4. Incubate 30 min at room temperature. Read OD30 at 340nm.5. Calculation:. Subtract OD0 from OD30 for the standard and sample wells. Use the DOD values to determine sample cholesterol concentration from the standard curve. Note: since both the standards and samples were diluted 10-fold, no dilution factor is required.Note: if the sample OD value is higher than OD for the 300 mg/dL standard, dilute sample in water and repeat the assay. Multiply the results by the dilution factor.Materials Required, But Not Provided:Pipetting (multi-channel) devices, clear bottom 96-well plate and plate reader.Examples:Samples were run in duplicate according to the standard procedure.The cholesterol concentrations (mg/dL) were 105 ± 3 for a human serum, 155 ± 11 for human plasma, 157 ± 2 for a bovine serum, 68 ± 2 for a rat serum, 129 ± 3 for a mouse serum, 123 ± 2 for a goat serum sample.
- 产品特点
- 针对Cholesterol Assay Kit, BioAssay, Colorimetric该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Cholesterol Assay Kit, BioAssay, Colorimetric产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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