- 中文名称
Cholesterol Assay Kit, BioAssay, Colorimetric/Fluorometric
- 英文名字
- Cholesterol Assay Kit, BioAssay, Colorimetric/Fluorometric
- 供应商
- USBiological
- 产品货号
- C5045-09
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage.Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. Cholesterol Assay uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex=585/530nm is directly proportional to total cholesterol concentration in the sample.Applications:Direct Assays: cholesterol in serum, plasma, and other biological samples.Pharmacology: effects of drugs on cholesterol metabolism.Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorometric assays.Convenient. Room temperature assay. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Kit Contents: (100 tests in 96-well plates)Assay Buffer: 20ml Enzyme Mix: 120uLDye Reagent: 120ul Standard: 1ml 300 mg/dL cholesterolStorage conditions. Store reagents at -20°C. Shelf life: 6 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Colormetric Procedure:Important: bring all reagents to room temperature prior to assay. Serum and plasma samples should be clear and free of turbidity or precipitates. If present, precipitates should be removed by filtration or centrifugation. If not assayed immediately, samples can be stored at -20 to -80°C for at least one year.1. Standard Curve. Prepare a 10-fold diluted 100mg/dL standard (STD) by mixing 15ul 300mg/dL Standard and 435ul Assay Buffer. Further dilute standard (STD) in Assay Buffer as shown below.No STD+Assay Buffer Vol (ul) 10 x Conc. (mg/dL)1 100ul+0ul 100 1002 80ul+20ul 100 803 60ul+40ul 100 604 40ul+60ul 100 405 30ul+70ul 100 306 20ul+80ul 100 207 10ul+90ul 100 108 0ul+100ul 100 0Transfer 50ul diluted standards into wells of a clear 96-well plate.Samples: dilute samples 10-fold (e.g. 10ul sample with 90ul Assay Buffer). Transfer 50ul diluted sample in separate wells.2. For each reaction well, mix 55ul Assay Buffer with 1ul Enzyme Mix and 1ul Dye Reagent. Add 50ul of this Working Reagent to each standard and sample well. Tap plate to mix well.3. Incubate 30 min at room temperature. Read OD at 570 nm.Note: if the Sample OD is higher than the Standard OD at 100 mg/dL, dilute sample in assay buffer and repeat the assay. Multiply result by the dilution factor.Calculation:Subtract blank OD (water, #8) from the standard OD values and plot the OD against standard concentrations. Determine the slope using linear regression fitting. The cholesterol concentration of Sample is calculated as[Cholesterol] =ODSample-ODBlankSlope(mg/dL)Note: since both the standards and samples were diluted 10-fold, no dilution factor is required.Fluorometric Procedure:For fluorometric assays, the linear detection range is 0.2 to 10 mg/dL cholesterol. Dilute the Standards prepared in Colorimetric Procedure: 1:10 in Assay Buffer.Transfer 50ul standards and 50ul samples into separate wells of a black 96-well plate.Add 50ul Working Reagent (see Colorimetric Procedure:). Tap plate to mix.Incubate 30 min at room temperature and read fluorescence at lex=530nm and lem=585nm.If assays in 384-well plate are desired, use 5ul Standards / samples and 45ul Working Reagent.The cholesterol concentration of Sample is calculated as[Cholesterol] =FSample-FBlankSlope(mg/dL)Materials Required, But Not Provided:Pipetting (multi-channel) devices, 96-well plate and plate reader.
- 产品特点
- 针对Cholesterol Assay Kit, BioAssay, Colorimetric/Fluorometric该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Cholesterol Assay Kit, BioAssay, Colorimetric/Fluorometric产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
