- 中文名称
Chloramphenicol, BioAssay ELISA Kit (Chloromycetin)
- 英文名字
- Chloramphenicol, BioAssay ELISA Kit (Chloromycetin)
- 供应商
- USBiological
- 产品货号
- C4350-06
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Due to its outstanding antibacterial properties, chloramphenicol is an often used antibiotic in the production of milk, meat and eggs. In humans it leads to haematotoxic side effects, like the chloramphenicol induced aplastic anemia. This has caused low limits in Germany, e.g. 1ug/kg for milk, meat and eggs.Till now the chloramphenicol concentration was determined by radioimmunoassay or by gas chromatography. However, compared with conventional methods, enzyme immunoassays show some essential advantages. There is no need to work with radioactive material, the required assay time is shorter and the sensitivity is better than with chromatographic methods.The United States Biological Chloramphenicol test provides a rapid, sensitive and reliable assay for the determination of chloramphenicol in food. 40 samples can be assayed in duplicate within 60 minutes.Intended Use:For the quantitative determination of chloramphenicol in food.Sensitivity: 0.03ng/mlRecovery (spiked samples): ~90-115%Incubation Time: 60 minTest Principle:The United States Biological Chloramphenicol quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a micro- titer plate. Chloramphenicol containing samples or standards and an antibody directed against chloramphenicol are given into the wells of the microtiter plate. The chloramphenicol contained in samples or standards will bind to the antibody which reacts with the binding protein coated onto the microtiter plate. After 30 minutes incubation at room temperature a chloramphenicol-peroxidase conjugate is added into the wells without a preceding washing step to saturate free antibody binding sites. After additional 15 minutes incubation at room temperature the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 15 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of chloramphenicol is indirectly proportional to the color intensity of the test sample.Kit Components:1. Microtiter plate 1x96 wells each, coated with an antibody binding protein.2. Standard 0ng/ml 1x1ml, ready to use.3. Standard 0.05ng/ml 1x1ml, ready to use.4. Standard 0.1ng/ml 1x1ml, ready to use.5. Standard 0.5ng/ml 1x1ml, ready to use.6. Standard 1ng/ml 1x1ml, ready to use.7. Standard 5ng/ml 1x1ml, ready to use.8. Anti-Chloramphenicol Antibody (sheep): 1x6ml, ready to use.9. Conjugate (Chloramphenicol-Peroxidase): 1x6ml, ready to use.10. Substrate Solution (TMB): 1x15ml, ready to use.11. Stop Solution (0.5 M H2SO4): 1x15m ready-to-use.12. Sample Diluent (PBS): 2x60ml, ready to use.13. Washing Solution 10x (PBS + Tween 20): 1x60ml. Dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up to 37°C for 15 minutes.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Chloramphenicol, BioAssay ELISA Kit (Chloromycetin)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Chloramphenicol, BioAssay ELISA Kit (Chloromycetin)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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