- 中文名称
Chemerin, ELISA BioAssay Kit (Retinoic Acid Receptor Responder Protein 2, RARRES2, Tazarotene-induced Gene 2 Protein, TIG2) discontinued
- 英文名字
- Chemerin, ELISA BioAssay Kit (Retinoic Acid Receptor Responder Protein 2, RARRES2, Tazarotene-induced Gene 2 Protein, TIG2) discontinued
- 供应商
- USBiological
- 产品货号
- C3890-03
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intented Use: This Human Chemerin ELISA kit is used for the non-radioactive quantification of Human Chemerin in serum, plasma, tissue extract and cell culture samples. One kit is sufficient to measure 38 unknown samples in duplicate.Principle of the Procedure:This assay is a Sandwich ELISA based, sequentially, on: 1) capture of Human Chemerin molecules from samples to the wells of a microtiter plate coated by a pre- titered amount of anti-Human Chemerin antibody, 2) wash away of unbound materials from samples, 3) binding of a biotinylated anti-human Chemerin antibody to the captured molecules, 4) wash away of unbound materials from samples, 5) conjugation of horseradish peroxidase to the immobilized biotinylated antibodies, 6) wash away of free enzyme conjugates, and 7) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate 3,3’,5,5’-tetramethylbenzidine. The enzyme activity is measured spectrophotometrically by the increased absorbency at 450 nm, corrected from the absorbency at 590 nm, after acidification of formed products. Since the increase in absorbency is directly proportional to the amount of captured Human Chemerin in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrations of Human Chemerin.Kit Components:Each kit is sufficient to run one 96-well plate and contains the following reagents:A. Human Chemerin ELISA PlateCoated with anti-Human Chemerin antibody Quantity: 1 strip plate Preparation: Ready to Use. Note: Unused strips should be resealed in the foil pouch with the dessicant provided and stored at 4°C.B. 10X HRP Wash Buffer Concentrate10X concentrate of 50 mM Tris Buffered Saline containing Tween-20. Quantity: 2 bottles containing 50 mL each Preparation: Dilute 1:10 with distilled or deionized water.C. Human Chemerin StandardHuman Chemerin, lyophilized. Quantity: 0.5 mL/vial upon hydration Preparation: Reconstitute with 0.5 mL distilled or deionized water.D. Human Chemerin Quality Controls 1 and 2 One vial each, lyophilized, containing Human chemerin at two different levels.. Quantity: 0.5 mL/vial upon hydration Preparation: Reconstitute with 0.5 mL distilled or deionized water.E. Assay Running Buffer, 0.05 Phosphosaline, pH 7.6 containing 0.025M EDTA, 0.08% Sodium Azide, 1.0% BSA Quantity: 2 bottles containing 10 mL each. Preparation: Ready to UseF. Serum Matrix Serum matrix solution, liquid Quantity: 0.5mL/vial Preparation: Add 0.5 mL of Assay Running Buffer into glass vial and invert and mix gently. The matrix solution is 1:2 dilutedG. Human Chemerin Detection Antibody, Pre-titered Biotinylated anti-human Chemerin Antibody Quantity: 12 mL Preparation: Ready to UseH. Enzyme Solution Pre-titered Streptavidin-Horseradish Peroxidase Conjugate in Buffer Quantity: 12 mL Preparation: Ready to UseJ. Substrate (Light sensitive, avoid unnecessary exposure to light) 3, 3’, 5, 5’-tetramethylbenzidine in buffer Quantity: 12 mL Preparation: Ready to Use.K. Stop Solution (Caution: Corrosive Solution)0.3M HCl Quantity: 12 ml Preparation: Ready to UseStorage and Stability:Recommended storage for kit components is 4°C. All components are shipped and stored at 4°C. Reconstituted standards and controls can be frozen for future use but repeated freeze thaws should be avoided. Refer to expiration dates on all reagents prior to use.
- 产品特点
- 针对Chemerin, ELISA BioAssay Kit (Retinoic Acid Receptor Responder Protein 2, RARRES2, Tazarotene-induced Gene 2 Protein, TIG2) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Chemerin, ELISA BioAssay Kit (Retinoic Acid Receptor Responder Protein 2, RARRES2, Tazarotene-induced Gene 2 Protein, TIG2) discontinued产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
