- 中文名称
Cell Cycle Checkpoint BioAssay Sample Kit (cdc2 (Tyr15), Chk1 (Ser345), Chk2 (Thr68), Rb (Ser795, Ser807/811), p53 (Ser15))
- 英文名字
- Cell Cycle Checkpoint BioAssay Sample Kit (cdc2 (Tyr15), Chk1 (Ser345), Chk2 (Thr68), Rb (Ser795, Ser807/811), p53 (Ser15))
- 供应商
- USBiological
- 产品货号
- C2612
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Synthetic peptides (KLH coupled) corresponding to residues surrounding PhosphorylatedTyr15 of human cdc2; Phosphorylated Ser345 of human Chk1; Phosphorylated Thr68 of human Chk2; and Phosphorylated Ser795 and Phosphorylated Ser807/811 of human Rb. Mab is produced by immunizing mice with a synthetic Phosphorylated Ser15 peptide (KLH coupled) corresponding to residues surrounding Phosphorylated Ser15 of human p53.Recognizes cdc2 Phosphorylated (Tyr15), Chk1 Phosphorylated (Ser345), Chk2 Phosphorylated (Thr68), Rb Phosphorylated (Ser795, Ser807/811) and p53 Phosphorylated (Ser15).The cell-replication cycle demands great accuracy in order to avoid the accumulation of genetic damage. This process is controlled by molecular circuits called “checkpoints” that are common to all eukaryotic cells (1). Checkpoints monitor DNA integrity and cell growth prior to replication and division at the G1/S and G2/M transitions, respectively. The cdc2-cyclin B kinase is pivotal in regulating the G2/M transition (2,3). cdc2 is phosphorylated at Thr14 and Tyr15 during G2-phase by the kinases Wee1 and Myt1, rendering it inactive. The tumor suppressor protein retinoblastoma (Rb) controls progression through the late G1 restriction point (R) and is a major regulator of the G1/S transition (4). During early and mid G1-phase, Rb binds to and represses the transcription factor E2F (5). The phosphorylation of Rb late in G1 by CDKs induces Rb to dissociate from E2F, permitting the transcription of S-phase genes. Rb can be phosphorylated at multiple sites in vitro by cdc2, cdk2 and cdk4/6 (6–8). DNA damage triggers both the G2/M and the G1/S checkpoints. DNA damage activates the DNA-PK/ATM/ ATR kinases, which phosphorylate Chk1 at Ser345, Chk2 at Thr68 and the tumor suppressor protein p53 (9–11). The Chk kinases inactivate cdc25 via phosphorylation at Ser216, blocking the activation of cdc2 and the transition into M-phase. The phosphorylation of p53 at Ser15 enables its dissociation from MDM2, activating its DNA binding activity. The Cell Cycle/Checkpoint Sampler Kit provides a fast and economical means to evaluate the activation status of multiple members of the cell cycle/ checkpoint signal, including cdc2 (Tyr15), Chk1 (Ser345), Chk2 (Thr68), Rb (Ser795, Ser807/811) and p53 (Ser15). The kit contains enough primary and secondary antibodies to perform four mini-blot experiments.Kit Components:C2612-01: cdc2 Phosphorylated (Tyr15) Pab 1x20ul Rabbit IgG 34kDC2612-02: Chk1 Phosphorylated (Ser345) Pab 1x20ul Rabbit IgG 56kDC2612-03: Chk2 Phosphorylated (Thr68) Pab 1x20ul Rabbit IgG 62kDC2612-04: Rb Phosphorylated (Ser795) Pab 1x20ul Rabbit IgG 110kDC2612-05: Rb Phosphorylated (Ser807/811) Pab 1x20ul Rabbit IgG 110kDC2612-06: p53 Phosphorylated (Ser15) Mab 1x20ul Mouse IgG 53kDC2612-07: IgG (HRP) 1x100ul Pab GtxRbRecommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
- 产品特点
- 针对Cell Cycle Checkpoint BioAssay Sample Kit (cdc2 (Tyr15), Chk1 (Ser345), Chk2 (Thr68), Rb (Ser795, Ser807/811), p53 (Ser15))该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Cell Cycle Checkpoint BioAssay Sample Kit (cdc2 (Tyr15), Chk1 (Ser345), Chk2 (Thr68), Rb (Ser795, Ser807/811), p53 (Ser15))产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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