- 中文名称
Caspase 3 Assay Kit, BioAssay
- 英文名字
- Caspase 3 Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- C2087-08
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction.Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (lexc/em=400/490nm) is proportional to the caspace-3 activity.Key Features:Safe. Non-radioactive assay.Convenient and high-throughput. Homogeneous mix-incubate-measure type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.Applications:Determination of caspase-3 activity in cell and cell lysate.HTS screening for apoptosis inducers and inhibitors.Kit Contents:Assay Buffer: 12ml Substrate: 240ulDTT Solution: 240ulStorage conditions: This product is shipped at room temperature. Upon delivery, store all reagents at -20°C. Shelf life of 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.96-Well Assay Procedure:Note: The following procedure is for standard assays in a 96-well plate. If cells are cultured in plates other than a 96-well plate, it is necessary to prepare the cell lysate (See General Considerations). Duplicate assays are recommended for controls and samples.1. Cell culture. Seed 100ul of 1,000 to 100,000 cells into wells of a sterile black clear-bottom 96-well plate (Note: The cell number to be used depends on the cell line). Incubate overnight at 37°C in a cell culture incubator.2. Cell treatment. Add 10ul test compounds (e.g. apoptosis inducers or inhibitors) at desired concentration, and 10ul vehicle (medium in which the test compound is dissolved) as a control. Incubate cells for a desired period of time.3. Caspase-3 Assay. Prior to assay, bring all reagents to room temperature. Briefly centrifuge tubes. Prepare enough Working Reagent for all assay wells. For each well, mix 100ul Assay Buffer, 2ul Substrate and 2ul DTT.Remove culture media from assay wells. For adherent cells, simply aspirate the culture media from wells. For suspension cells, centrifuge cells for 5 min at 500 x g, carefully remove media by aspiration. Immediately add 100ul Working Reagent to each assay well. Mix the reagents completely by shaking the plate for 60 sec at 100-200 rpm on a plate shaker. The Working Reagent lyses cells and supports optimal caspase-3 activity. Incubate plate at 37°C for 60 min in the dark.4. Read fluorescence intensity at lexc/em=400/490 nm.Note: If the caspase-3 activity is low, increase the incubation time, or use more cells.Calculation:Subtract the fluorescence intensity values from that of the control wells. The DF values represent the relative caspase-3 activity.General Considerations:For cells not cultured in a 96-well plate, cell lysates are prepared separately and used for the caspase assay. After cells have been treated with test compounds for the desired period of treatment, remove culture medium. For adherent cells simply aspirate the culture medium. For suspension cells centrifuge cells at 500 x g for 5 min and aspirate the medium. Lyse cells by adding 300ul per 106 cells of 50mM Hepes (pH 7.2), 100mM NaCl, 0.5 % (v/v) Triton X-100. Shake the cell suspension on for 30 min at 4°C. Centrifuge the cell suspension at 2,500 x g for 10 min at 4°C. Transfer supernatants to clean tubes. If not assayed on the same day, lysates can be stored at -80°C for one month. To assay, add 50ul of sample lysates into separate wells of a black 96-well plate. Immediately add 100ul Working Reagent to each assay well. Incubate plate at 37C for 60 min in the dark. Read fluorescence intensity lexc/em=400/490 nm.Materials Required, But Not Provided:Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader.
- 产品特点
- 针对Caspase 3 Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Caspase 3 Assay Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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