- 中文名称
Casein Kinase 2 Assay Kit, RRRDDDSDDD, BioAssay (CK2) discontinued
- 英文名字
- Casein Kinase 2 Assay Kit, RRRDDDSDDD, BioAssay (CK2) discontinued
- 供应商
- USBiological
- 产品货号
- C2085-64
- 产品报价
- ¥1/1Kit

- 产品说明书
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- 购买方式
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- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- The assay kit is designed to measure the phosphotransferase activity of Casein Kinase-2 (CK-2) in immunoprecipitates and column fractions. Crude cell lysates may also be used but detergents or biochemicals contained in the cell lysis buffer may inhibit CK-2 activity. Furthermore, although an inhibitor cocktail is included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for CK-2 substrate phosphorylation. The assay kit is based on phosphorylation of a specific substrate (CK-2 substrate peptide) using the transfer of the gamma-phosphate of [g-32P]ATP by CK-2 kinase. The phosphorylated substrate is then separated from the residual [g-32P]ATP using P81 phosphocellulose paper and quantitated by using a scintillation counter. The assay is linear for incubation times of up to 30 minutes and incorporation of up to 20% of total ATP. Further incubation or incorporation may not be linear and may therefore not be a true indication of CK-2 activity in the sample extract. The enzyme assay is rapid, convenient and fairly specific for CK-2. Each kit contains sufficient reagents for 200 individual CK-2 assays. detection and quantitation of casein kinase 2 activity from cell extracts.Quantity: ~200 assaysKit Components: C2085-64A: Assay Dilution Buffer 2x1mlC2085-64B: CK2 Substrate Peptide 2x1mlC2085-64C: PKA Inhibitor Cocktail 2x1mlC2085-64D: Mg/ATP Cocktail 2x1mlC2085-64E: p81 pre-labeled squares 200Storage and Stability:Store at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
- 产品特点
- 针对Casein Kinase 2 Assay Kit, RRRDDDSDDD, BioAssay (CK2) discontinued该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Casein Kinase 2 Assay Kit, RRRDDDSDDD, BioAssay (CK2) discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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