- 中文名称
Catenin, beta, BioAssay ELISA Kit (Human)
- 英文名字
- Catenin, beta, BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- C2069-38
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- A key downstream effector in the Wnt signaling pathway is b-catenin (1). It is implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). Casein kinase 1 phosphorylates b-catenin on Ser45. This phosphorylation event primes b-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3b destabilizes b-catenin by phosphorylating it at Ser33, 37 and Thr41 (7). Mutations in these phosphorylation sites which result in the stabilization of b-catenin protein levels have been found in many tumor cell lines (8).This ELISA Kit is a solid phase sandwich enzyme-linked im munosorbent assay (ELISA) that detects endogenous levels of total beta-catenin protein (phosphorylated and non-phosphorylated). A beta-Catenin antibody has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-beta-catenin proteins are captured by the coated antibody antibody. Following extensive washing, beta-Catenin mouse monoclonal antibody is added to detect both the captured phospho- and nonphospho-beta-catenin protein. An anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color color. The magnitude of optical density for this developed color is proportional to the quantity of total beta-catenin protein.Kit Components:1. Microtiter Plate, Catenin antibody-coated microtiter, 1x96 wells. 2. Beta-Catenin Detection Ab, 1x11ml3. IgG (HRP) Ab, 1x11ml 4. TMB Substrate, 1x 11ml5. STOP Solution, 1x11ml6. Wash Buffer (20X), 1x25ml7. Sample Diluent, 1x25ml.8. Cell Lysis Buffer, 1x15mlStorage and Stability:Store all kit components at 4°C except Cell Lysis Buffer. Store Cell Lysis Buffer at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Catenin, beta, BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Catenin, beta, BioAssay ELISA Kit (Human)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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