- 中文名称
Campylobacter Antigen (In Food) BioAssay ELISA Kit
- 英文名字
- Campylobacter Antigen (In Food) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- C1037-19
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Infection by thermophilic Campylobacter species is a leading cause of human gastroenteritis. Of the various species of Campylobacter, C. jejuni, C. coli and C. lari, are the species most often associated with human illness. Campylobacter are often passed to humans through the handling or consumption of contaminated food, particularly foods of animal origin.Recently, human infection with Campylobacter has been implicated in the induction of Guillain- Barré Syndrome (GBS) and reactive arthritis. GBS is a debilitating and potentially fatal neurological disease that produces paralysis.Campylobacter species are gram negative, motile curved or spiral rods that require highly specialized growth conditions. Typical cultivation entails pre-enrichment and enrichment steps in broth, followed by isolation on a selective solid medium. Of particular importance in the cultivation of Campylobacter is the requirement for a microaerobic atmosphere.Cultivation of thermophilic Campylobacter species from foods involves multiple media steps, and thus many days. The United States Biological Campylobacter Microwell ELISA allows rapid testing (less than an hour) of the enrichment broth, thus eliminating the need for the plating and identification steps used in the standard methods. The United States Biological Campylobacter Microwell ELISA reduces time to result by several days.Intended Use:The Campylobacter Antigen (In Food) ELISA Kit is an enzyme-linked immunosorbent assay that may be used to screen food products for the presence of thermophilic Campylobacter antigen.Principle of the Test:The Campylobacter Antigen (In Food) ELISA is a double antibody (sandwich) assay that uses specific anti-Campylobacter antibodies coated to microwells. After addition of the sample and the enzyme conjugate, a positive reaction (indicating the presence of Campylobacter antigen) produces a deep blue color. Addition of the Stop Solution ends the assay and turns the blue color to yellow. The results may be read visually or with an ELISA reader.Kit Components:C1037-19A: Microwell test strips, 1x96 wells and supplied with one holder. Coated with anti-Campylobacter polyclonal antibody. C1037-19B: Enzyme Conjugate, 1x11ml. Peroxidase conjugated anti-Campylobacter polyclonal antibody with red dye and a preservative.C1037-19C: Positive Control, 1x2ml. Campylobacter antigen in a buffered base.C1037-19D: Negative Control, 1x2ml. Buffered base.C1037-19E: Chromogen, 1x11ml. Tetramethylbenzidine (TMB).C1037-19F: Wash Concentrate (20X), 2x25ml. Concentrated buffer and surfactanct with preservative.C1037-19G: Stop Solution, 1x11ml. Phosphoric Acid.Storage and Stability:Store reagents, strips and bottled components at 4°C. Unopened kit is stable for 6 months upon receipt.Test Procedure:1. Break off the required number of wells (number of samples plus 2) and place in strip holder. 2. Add 100ul of the negative control to well #1 and 100ul of the positive control to well #2. 3. Add 100ul of the test sample to the appropriate well. 4. Incubate at room temperature (15 to 25°C) for 30 minutes, then wash. Each washing consists of dumping the contents of the wells into an appropriate container with disinfecting solution (e.g. 3% bleach in water) and using the diluted wash buffer to fill in each well, shaking out the contents and refilling the wells for a total of 3 times. Samples with sticky particulate matter may require more thorough washing than other samples. The potential exists for false positive results if the sample is not thoroughly washed from the well before addition of subsequent reagents.5. Add 2 drops of Enzyme Conjugate (red solution) to each well.6. Incubate for 15 minutes, then wash. Slap out excess fluid against an absorbent towel. 7. Add 2 drops of Chromogen to each well. 8. Incubate for 5 minutes. 9. Add 2 drops of stop solution to each well. Mix wells by gently tapping the side of the strip holder with index finger. 10. Read results visually or at 450/620-650 nm.Interpretation of Results –Visual:Positive: Any sample that has significant and obvious yellow color.Negative: Any sample that does not have significant and obvious yellow color.Interpretation of Results – OD Readings:Zero reader on air. Read all wells using a bichromatic reading with filters at 450nm and 620- 650nm.Positive: Add 0.10 OD unit to the value of the negative control. Sample OD values of 0.10 above the negative control indicates the sample contains Campylobacter antigen. Negative: Absorbance reading less than 0.10 plus the negative control indicates the sample does not contain detectable levels of Campylobacter antigen.
- 产品特点
- 针对Campylobacter Antigen (In Food) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Campylobacter Antigen (In Food) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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