- 中文名称
Calpain, Fluorometric BioAssay Kit
- 英文名字
- Calpain, Fluorometric BioAssay Kit
- 供应商
- USBiological
- 产品货号
- C1035-31B
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity. The Extraction Buffer provided with the kit specifically extracts cytosolic proteins without contaminations of cell membrane and lysosome proteases. The Extraction Buffer also prevents auto-activation of calpain during the extraction procedure. Thus, the kit detects only activated calpain in cytosol upon treatment of cells with inducers (e.g., chemicals or drugs). The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (lmax=400nM); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (lmax=505nM), which can be quantified using a fluorometer or a fluorecence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.Intended Use:The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 505nm using a fluorometer or a fluorecence plate reader.Sample Type:Cell and tissue lysatesSpecificity:MammalianAbsorption: 400nmExcitement: 505nmFluorescence: Ex/Em 400/505nm Kit Components: *C1035-31B1: Extraction Buffer, 1x25ml*C1035-31B2: Reaction Buffer 10X, 1x1.5mlC1035-31B3: Calpain Substrate Ac-LLY-AFC, 1x500ul C1035-31B4: Active Calpain I (Positive Control), 1x10ulC1035-31B5: Calpain Inhibitor Z-LLY-FMK, 1x10ulStorage and Stability:Store components at -70°C. Once *C1035-31B1 and *C1035-31B2 are opened, store at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Calpain, Fluorometric BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Calpain, Fluorometric BioAssay Kit产品后将其置于-70°C/4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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