- 中文名称
Calcium Assay Kit, BioAssay
- 英文名字
- Calcium Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- C0115-89L
- 产品报价
- ￥1/500Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Calcium is measured to monitor diseases of the bone or calcium regulation disorders. Increased calcium levels in serum are reported in hyperparathyroidism, metastatic bone lesions and hypervitaminosis, while decreased levels are observed in hypoparathyroidism, nephrosis, rickets, steatorrhea, nephritis and calcium-losing syndromes. Urinary calcium levels aid the clinician in understanding how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the medical evaluation of kidney stones.Simple, direct and automation-ready procedures for measuring calcium concentration in biological samples are becoming popular in Research and Drug Discovery. Calcium assay kit is designed to measure calcium directly in biological samples without any pretreatment. A phenolsulphonephthalein dye in the kit forms a very stable blue colored complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipid, protein and bilirubin.Key Features:Sensitive and accurate. Use as little as 5ul samples. Linear detection range 0.08 mg/dL (20uM) to 20 mg/dL (5mM) Ca2+ in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc.Applications:Direct Assays: Ca2+ in serum, urine, saliva etc.Drug Discovery/Pharmacology: effects of drugs on calcium metabolism.Food and Beverages: calcium determination.Environment: calcium determination in water and soil.Kit Contents: (500 tests in 96-well plates)Reagent A: 50ml Reagent B: 50mlCalcium standard: 1ml 20 mg/dL Ca2+Storage conditions. The kit is shipped at room temperature. Store Reagent and Standard at 4°C. Shelf life: 12 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Prepare enough working reagent by combining equal volumes of Reagent A and Reagent B. Equilibrate to room temperature before use.Procedure using 96-well plate:1. Dilute standards as follows. Transfer 5ul diluted standards and samples into wells of a clear bottom 96-well plate. Store diluted standards at 4°C for future use.No STD+H2O Vol (ul) Ca (mg/dL)1 100ul+0ul 100 202 80ul+20ul 100 163 60ul+40ul 100 124 40ul+60ul 100 85 30ul+70ul 100 66 20ul+80ul 100 47 10ul+90ul 100 28 0ul+100ul 100 02. Add 200ul working reagent and tap lightly to mix.3. Incubate 3 min at room temperature and read optical density at 570-650nm (peak absorbance at 612nm).Procedure using cuvette:1. Set up test tubes for diluted standards and Samples. Transfer 15ul diluted Standards and samples to appropriately labeled tubes.2. Add 1000ul working reagent and vortex to mix. Incubate 3 min. Transfer to cuvet and read optical density at 612nm.Calculation:Subtract blank OD (water, #8) from the standard OD values and plot the OD against Ca2+ standard concentrations. Determine the slope using linear regression fitting. Calcium concentration of the sample is calculated as=ODSAMPLE–ODBLANKSlope(mg/dL)ODSAMPLE and ODBLANK are OD612nm values of sample and sample blank (water or buffer in which the sample was diluted).Conversions: 1 mg/dL Ca2+ equals 250uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices and accessories (e.g. 5ul).Procedure using 96-well plate:Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.Procedure using cuvette:Cuvets and Spectrophotometer for measuring OD612nm.General Considerations:EDTA and other Ca2+ chelators interfere with this assay. This assay can not be applied to plasma samples obtained with EDTA.Examples:Samples were assayed in duplicate using the 96-well plate protocol. The Ca2+ values (mg/dL) were 8.5 ± 0.4 (rat serum), 6.5 ± 0.3 (human serum), 7.6 ± 0.1 (goat serum), 11.1 ± 1.0 (fetal bovine serum), 2.5 ± 0.4 (fresh human urine), 41.3 ± 0.5 (Kirkland 2% reduced fat milk), 5.0 ± 0.0 (tap water, Hayward, CA), 0.86 ± 0.07 (tap water, San Bruno, CA), 1.8 ± 0.1 (Crystal Geyser natural alpine spring water), 2.3 ± 0.1 (Coca-cola® classic coke), 0.04 ± 0.01 (Lipton Lemon iced tea), 0.52 ± 0.07 (soil extract. 5.6 g of Hayward, CA soil was extracted with 10ml MilliQ water. The supernatant was centrifuged to remove any insoluble particles. Clear supernatant was assayed).
- 产品特点
- 针对Calcium Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Calcium Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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