- 中文名称
EvaGreen®，2000 X在DMSO中
- 英文名字
- EvaGreen®, 2000X in DMSO
- 供应商
- Biotium
- 产品货号
- BTM-31019
- 产品报价
- ￥询价/50UL
- 产品说明书
- 点击查看
- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- EvaGreen® Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution. Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not™ EvaGreen® qPCR Master Mixes or EvaGreen® Dye, 20X in water. EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer. The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis. EvaGreen® dye and applications are covered under granted and pending US and international patents.
- 产品描述
- EvaGreen®Dye，2000 X in DMSO适用于需要浓缩染料溶液的特殊应用。& nbsp; 注意：对于大多数qPCR应用，我们建议使用即用型勿忘我™EvaGreen®qPCR Master Mixes或EvaGreen®Dye，20 X水溶液。& nbsp; EvaGreen®染料是一种绿色荧光核酸染料，其特性使该染料可用于多种应用，包括qPCR和DNA熔解曲线分析、嗜热解旋酶依赖性扩增（tHDA）的实时监测和毛细管凝胶电泳。染料本身基本上是非荧光的，但在与dsDNA结合时变得高度荧光。DNA结合的染料具有非常接近荧光素（FAM）或SYBR绿色I的激发和发射光谱®，使其容易与配备有488 nm氩激光器或具有该区域波长的任何可见光激发的仪器兼容。EvaGreen®染料在热和水解方面都非常稳定，在常规处理过程中提供方便。EvaGreen®染料是非致突变性和非细胞毒性的，因为它是不可渗透的细胞膜，不像SYBR®Green I，它迅速进入细胞，是一种强大的突变增强剂。nbsp; EvaGreen染料的独特性质®使其在定量实时PCR（qPCR）应用中特别有用。与广泛使用的SYBR®绿色I相比，EvaGreen®染料通常对PCR的抑制性较小，并且不太可能引起非特异性扩增。因此，EvaGreen®染料可以以比SYBR Green I高得多的染料浓度使用®，从而产生用于液滴数字PCR、实时PCR和熔解曲线分析的高分辨率信号。nbsp; EvaGreen®染料和应用包括在已批准和正在申请的美国和国际专利中。
- 产品形式
- 该EvaGreen®，2000 X在DMSO中产品形式待更新请参考产品说明书
- 保存建议
- 室温
- 其他
- Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAY AREA------中外知名首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生 物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多，包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料Gel Red和GelGreen，以及最新推出的Cheetah Taq热启动酶和CF系列蛋白质标记染料等。 n目前，Biotium已将其许多专利技术授权给一些主要的国际生物技术公司，包括安捷伦、BD生物科学、Biolegend、BioRad、EMD Millipore、Illumina、PerkinElmer、Qiagen、Roche、Sartorius AG、Sigma-Aldrich、Thermo Fisher Scientific等。
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
