Luciferase assay HTS Kit w/o disp. is suitable when a luminometer without dispensers is available and you want to perform an HTS assay. The kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays: 1) Firefly luciferase is not present in normal cells. 2) The assay is simple and easy to perform. 3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers. 4) It is suitable for HTS. The kit contains reagents for 4800 assays. If you want to perform the assay in a smaller scale, the Luciferase Assay Kit 484-001 is suitable. Luciferase assay HTS Kit w/ disp. 484-103 is suitable if you want to perform the assay as HTS and have a luminometer with dispensers.
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Luciferase assay HTS Kit w/o disp. is suitable when a luminometer without dispensers is available and you want to perform an HTS assay.nThe kit contains reagents for 4800 assays. Detection limit: With most luminometers 10 -19 moles of luciferase can be detected.
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BioThema成立于1987 年,总部位于瑞典斯德哥尔摩南郊,只做一件事:把萤火虫荧光素酶反应做到极致。38 年来,公司依旧保持“小而专”的规模,却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测,包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商;