484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:nn1) Firefly luciferase is not present in normal cells.nn2) The assay is simple and easy to perform.nn3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.nn4) It is suitable for HTS.nnThe kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.nThe firefly luciferase luc gene is the most commonly used reporter gene for studying cellular events coupled to gene expression. It has a number of distinct advantages such as: no background, easy analytical procedure, extreme sensitivity (10-20 mole luciferase can be detected) and reliability of results. The reagents contain special activators to counteract any inactivation of the luciferase enzyme during cell culture or sample storage. All three Luciferase Assay Kits contain the same reagents. The High Throughput Screening kits (484-102 and 484-103) come in larger vials for easier handling.
产品描述
Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression.nnDetection limit:nnWith most luminometers 10 -19 moles of luciferase can be detected.
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BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；
