ATP Standard 10 nM is diluted in Extractant B/S and is used in hygiene control assays. The ATP Standard is stable in liquid form and certified by the Swedish Standards Institute.nnATP measurements are like most measurements subjects to inhibition and other variables. These parameters could be known or unfamiliar to the user and their significance may vary between measurements.nnThe RLU output from the same amount of ATP will vary with temperatur, pH, ATP reagents, different Luminumeters and very often with the sample matrix.nnCalibration with ATP standard addition (i.e know amount of ATP) accounts for:n- Variations between luminometers, pH and temperaturen- Inhibition or quenching of light from the samplen- Differences in the sensitivity of reagentsnnnAs a result the the usage of ATP standard makes it possible to convert the RLU result of your sample to amounts of ATP (pmol). The ATP Standard is delivered as a stable solution of known concentration. The method is very easy and rapid. Immediately after you take the light reading from your sample, just add the ATP standard to your sample and measure the light emitted. The amount of ATP in your sample can be simply calculated as follows:nnATP smp = 1 x I smp / ( I smp + std – I smp )nnI smp is the light emission from the sample I smp + std is the light emission from the sample plus ATP standard The factor 1 is the amount of ATP Standard added (i.e. 1 pmol).nnInstructions on the use of the ATP Standard accompany each assay kit.
产品描述
ATP standard makes i possible to express ATP measurements in moles rather than RLU.nATP Standard 10 nM is diluted in Extractant B/S and is used in hygiene control assays. The ATP Standard is stable in liquid form and certified by the Swedish Standards Institute.nnDelivery form:nSingle reagentnnNumber of assays:n6,7 mL allows up to approx. 220 assays.nnStorage:nAt +4 °C or lower.nnShelf life:n2 years from date of delivery at +4 °C.
产品形式
保存建议
4 °C
其他
BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；
