中文名称

ATP 生物质试剂盒 HS

英文名字

ATP Biomass Kit HS

供应商

BioThema

产品货号

BTA-266-311

产品报价

￥询价/375tests

产品说明书

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购买方式

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产品新闻

背景资料

This kit is intended for determination of total ATP (adenosine triphosphate) in samples containing living cells. If the intention is to determine intracellular ATP one should consider using 266-111 Intracellular ATP Kit HS or 266-112 Microbial ATP Kit HS. These kits include an ATP Eliminating Reagent for degradation of extracellular ATP and the latter kit also a Cell Lysing Reagent for lysis of most animal cells. All living cells contain ATP where it plays the role of energy currency between different cellular processes.nnWhen cells die of natural causes, ATP is normally degraded. The intracellular concentration of ATP is carefully regulated to similar levels in all types of cells. ATP is therefore a good estimate of the total intracellular volume. Most bacterial cells contain approx. 2*10-18 mol ATP per cell, while most eukaryotic cells, as a result of their larger size, contain 10 -15 mol ATP or more. ATP Biomass Kit HS, Intracellular ATP Kit HS and Microbial ATP Kit HS contain ATP Reagent HS (High Sensitivity) with a high luciferase activity. The luciferase activity in ATP Reagent HS is actually so high that if contaminated during reconstitution it will degrade the ATP background by one order of magnitude every 38 min. After mixing sample and ATP Reagent HS the light intensity decays by approx. 6% per min.nnThis is slow enough to allow manual mixing and a tube luminometer without reagent dispenser can be used. The detection limit of the reagent is 10*10-18 mol ATP with a sensitive luminometer. This corresponds to 5 bacterial cells. We must here differentiate between bacterial cells and CFU, since one CFU may consist of several cells. If the detection limit is not adequate, it is possible to concentrate the sample by microfiltration, a procedure that will also remove most of the extracellular ATP.

产品描述

The ATP Biomass Kit is suitable for virtually all types of samples.nThe sensitivity of this kit allows determination of low ATP levels in a variety of circumstances:n1. Liquids (e. g. drinking water, process water, environmental samples, beverages and beer)n2. Pharmaceuticals and cosmeticsn3. Cell adhesion to surfaces (e. g. biocompatibility studies)n4. Quality assurance of laundryn5. Bacterial growth.n6. Antibiotic effects on bacteriannNumber of assays:nThe kit contains reagents for 150-375 assays (each vial of ATP Reagent HS is sufficient for 25 assays in a tube luminometer and 62.5 assays in a microplate luminometer).nnDetection limit:nThe detection limit of the reagent is 10×10 -18 mol ATP with a sensitive luminometer. This corresponds to 5 bacterial cells.

产品形式

保存建议

其他

BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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