Cell Viability Kit SL is intended for cell proliferation and cytotoxicity studies by determination of total ATP (adenosine triphosphate). Extractant B/S included in the kit releases ATP from most types of cells and inactivates ATP degrading enzymes. For maximum accuracy, the level of extracellular ATP should be low compared to the intracellular level.nnWith low numbers of cells, ATP degrading enzymes are not a problem and 155-050 Cellular ATP Kit HTS can be used for mammalian but not microbial cells. This kit allows a mix-and-measure assay with only one step (the Cell Viability Kit SL requires two steps – first extraction and then light measurement). If a higher sensitivity is required or with bacterial samples, the 266-311 ATP Biomass Kit HS, 266-111 Intracellular ATP Kit HS or 266-311 Microbial ATP Kit HS are recommended. Assays of ATP replace methods such as tritiated thymidine incorporation and tetrazolium salt reduction.nnThe Cell Viability Kit SL is useful for quantification of ATP in range of 10-11 – 10-7 mol/L. This dynamic range covers the commonly used seeding cell number and culturing media.nnAll living cells contain ATP where it plays the role of energy currency between different cellular processes. When cells die of natural causes, ATP is normally degraded. The intracellular concentration of ATP is carefully regulated to similar levels in all types of cells. ATP is therefore a good estimate of the total intracellular volume. Most bacterial cells contain approx. 2*10-18 mol ATP per cell, while most eukaryotic cells, as a result of their larger size, contain 10-15 mol ATP or more.nnThis kit can be used for determination of ATP levels in e.g. animal or bacterial cells in a variety of circumstances:nn1. Estimation of cell numbersnn2. Cell proliferation studiesnn3. Cytotoxicity studies
产品描述
Cell Viability Kit SL is intended for use with mammalian cell samples with high cell density or with cell samples with higher density of ATPases, e.g. nerve cells. The kit contains a stronger ATPase inhibitor than 155-050 Cellular ATP Kit HTS and produces stable light.nA mammalian cell contains approx. 1 femtomole ATP. 188-441 Cell Viablity Kit SL is useful for quantification of ATP in the range 10-11 – 10-7 mole/L.nNumber of tests:n320-1600 tests depending on whether assays are performed in cuvettes or microplates.nnDetection limit:nQuantitative detection of ATP in range of 10-11 – 10-7 mol/L.
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BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；
