Cellular ATP Kit HTS is intended for enumeration of viable cells by quantification of cellular ATP (adenosine triphosphate). The assay is intended for rapid analysis of cytotoxicity and proliferation in cell studies. The assay of cellular ATP replaces methods such as tritiated thymidine incorporation and tetrazolium salt reduction. If a stable light emission is not obtained, the ATPases are not completely inhibited. We then recommend the Cell Viability Kit SL containing a strongly ATPase inactivating agent.nnThe Cellular ATP Kit HTS is especially designed for HTS screening methodology; enabling assay in 96 well format with manual or fully automated handling. The assay format is a mix and measure addition of ATP Reagent containing cellular lysis agent and stabilizers for the firefly luciferase enzymatic reaction. The T1/2 of the light emission is 3 hours in medium containing 10% FCS (Fetal Calf Serum) and 12.5 hours in media supplemented with e.g. vitamin mixture F12, enabling screening of a large number of samples. The Cellular ATP Kit HTS is useful for quantification of ATP in range of 10-11 – 10-7 mol/L. This dynamic range covers the commonly used seeding cell number and culturing media.nnIn samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.
产品描述
Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.nNumber of assays:n1200 tests depending on whether assays are performed in microplates.nnDetection limit:nQuantitative detection of ATP in the range of 10–11 to 10–7 mol/L.
产品形式
保存建议
其他
BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；
