ATP Reagent SL-M is specially developed for the assay of mithocondrial ATP metabolism, monitoring of oxidative phosphorylation with different substrates as described in Wibom, Lundin & Hultman (1990) Scand J Clin Lab Invest 50:143-152. The reagent can be used for monitoring adenosine triphosphate (ATP) over the range 10-12 – 10-6 mol/L. In contrast to our normal ATP Reagent SL 11-501 it does not contain magnesium ions. This makes the reagent useful for situations when the concentration and counterion of the magnesium salt used in the normal reagent is not optimal. The reagent has a decay of ATP and light emission of less than 1% per minute. The stable light allows manual handling and you can use a simpler luminometer. The detection limit is 10-15 mole ATP, making the reagent suitable for assays of mithocondrial ATP production rate down to the detection limit and for other continous monitoring of enzymatic reactions where the magnesium ion concentration has to be adjusted.
产品描述
The detection limit is 10-15 mole ATP, making the reagent suitable for assays of mithocondrial ATP production rate down to the detection limit and for other continous monitoring of enzymatic reactions where the magnesium ion concentration has to be adjusted.nnDelivery form: Lyophilised reagent in a glass vial, sealed under argonnnReconstitution: In 23-101 Diluent C. A further 5 fold dilution in Tris-EDTA buffer or other suitable buffer is recomended, for maximum performance.nnNumber of assays: Up to 250 analyses in a 96 well microplate format or 50 analyses in cuvette format.nnLinear range: From 10-15 mole to 10-12 mole ATP, depending on the linearity of the luminometer.nnStorage: +4°CnnShelf life: 2 years from date of delivery at +4°C. Reconstituted reagent should be used within a week.
产品形式
保存建议
4 °C
其他
BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；
