Substrate-recognition component of a DCX (DDB1-CUL4-X-box E3 ubiquitin-protein ligase complex involved in cell cycle control and autophagy. The DCX(AMBRA1 complex specifically mediates the polyubiquitination of target proteins such as BECN1, CCND1, CCND2, CCND3, ELOC and ULK1. Acts as an upstream master regulator of the transition from G1 to S cell phase: AMBRA1 specifically recognizes and binds phosphorylated cyclin-D (CCND1, CCND2 and CCND3, leading to cyclin-D ubiquitination by the DCX(AMBRA1 complex and subsequent degradation. By controlling the transition from G1 to S phase and cyclin-D degradation, AMBRA1 acts as a tumor suppressor that promotes genomic integrity during DNA replication and counteracts developmental abnormalities and tumor growth.
应用类型
WB
免疫原
Recombinant fusion protein of human Ambra1(NP_060219.2).
