l across the ER membrane. A human homolog of this kinase, IRE1α, was later identified and shown to be ubiquitously expressed in human tissues. Upon activation of UPR, IRE1α splices X-box binding protein (XBP1) mRNA by an unconventional mechanism using its endoribonuclease activity. This converts XBP1 into a potent transcriptional activator that induces many UPR responsive genes. Recently, IRE1α was shown to mediate the rapid degradation of certain mRNAs based on the ER-localization and primary sequences of their encoded proteins, suggesting a novel mechanism in UPR.
应用类型
WB, IHC, IF/ICC
免疫原
A synthetic phosphorylated peptide around S724 of human IRE1 (NP_001424.3).
