- 中文名称
人白介素6 Elisa试剂盒
- 英文名字
- Human IL-6 ELISA Kit
- 供应商
- Biomedica Immunoassays
- 产品货号
- BI-IL6
- 产品报价
- ￥12x8tests
- 产品说明书
- 点击查看
- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- Interleukin-6 (IL-6), also known as B-cell stimulatory factor 2 (BSF-2), CTL differentiation factor (CDF), Hybridoma growth factor or Interferon beta-2 (IFN-beta-2), was successfully cloned by Hirano et al. in 1986 (Hirano T et al.) . The gene is mapped at chromosome 7p21. IL-6 protein is built up by 183 amino acids and has a calculated molecular weight of 23.7 kDa.
It is a pleiotropic, alpha helical protein that is composed of a four-helix bundle (Somers W et al.). It shares 39% sequence identity with mouse and 40% with rat IL-6. IL-6 is phosphorylated at amino acid 81 and it is variably glycosylated by N-linked glycosylation. IL-6 belongs to the IL-6/GCSF/MGF protein family (Rose-John S et al.) whose members share a common use of the gp130 receptor subunit. IL-6 isoforms, with internal deletions, are generated by alternative splicing. The principal cell sources for IL-6 are mononuclear phagocytes, vascular endothelial cells, fibroblasts or other cells.
IL-6 is the ligand for the Interleukin-6 receptor α (IL-6Rα) (Schwantner A et al.) that occurs membrane-bound, but that may also circulate as soluble form generated by alternative splicing or proteolytic cleavage. To induce signaling, IL-6 first forms a complex with the non-signaling IL-6Rα. Subsequent binding to the signal transducing subunit gp130 leads to dimerization of gp130 and finally to the formation of the hexameric signaling complex (Boulanger MJ et al.).
Complexes of IL-6 and soluble IL-6Rα may elicit responses in cells lacking the membrane-bound IL-6Rα but expressing the ubiquitous gp130 coreceptor. This process is known as trans-signaling, it enlarges the spectrum of target cells responding to IL-6 (Mihara M et al.).
- 产品描述
- Method
Sandwich ELISA, HRP/TMB, 12×8-well detachable strips
Sample type
Serum, plasma (EDTA, citrate, heparin), cell culture supernatants, urine
Sample volume
100 µl sample / well
Assay time
2 h / 1 h / 1h /30 min
Sensitivity
LOD: 0.28 pg/ml; LLOQ: 0.78 pg/ml (measurable concentrations in serum AND plasma samples!)
Standard range
0 – 200 pg/ml (0 / 3.12 / 6.25 / 12.5 / 25/ 50 / 100 / 200)
Precision
In-between-run (n=3): ≤ 6 % CV
Within-run (n=3): ≤ 7 % CV
Specificity
This assay recognizes recombinant and endogenous (natural) human IL-6
Validation Data
See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc
- 产品特点
- 保存建议
- +4°C
- 其他
- Biomedica Immunoassay(bmgrp)公司成立于1988年, 总部位于奥地利维也纳，主要致力于研究、开发和生产基于ELISA方法用于临床前研究相关标记物检测的工具。迄今为止Biomedica Immunoassay公司在心血管检测及骨代谢检测领域开发了一系列特色ELISA检测试剂盒，Biomedica Immunoassay的生产过程符合GMP/GLP指南，并符合ISO 9001:2015管理体系，有CE认证。Biomedica Immunoassay的产品根据FDA， EMEA和ICH指南进行验证，并具有验证报告。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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