欢迎您访问武汉艾美捷科技有限公司官方网站!
站点地图 服务热线:400-6800-868
  • 产品
  • 文章

PRODUCT CENTER

ELISA试剂盒
产品中心

当前位置:首页 > 产品中心 > 免疫学产品 > ELISA试剂盒

中文名称

人白介素6 Elisa试剂盒

英文名字
Human IL-6 ELISA Kit
供应商
Biomedica Immunoassays
产品货号
BI-IL6
产品报价
¥12x8tests
产品说明书
点击查看
购买方式
90%产品中国有现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868
产品新闻
背景资料
Interleukin-6 (IL-6), also known as B-cell stimulatory factor 2 (BSF-2), CTL differentiation factor (CDF), Hybridoma growth factor or Interferon beta-2 (IFN-beta-2), was successfully cloned by Hirano et al. in 1986 (Hirano T et al.) . The gene is mapped at chromosome 7p21. IL-6 protein is built up by 183 amino acids and has a calculated molecular weight of 23.7 kDa.

It is a pleiotropic, alpha helical protein that is composed of a four-helix bundle (Somers W et al.). It shares 39% sequence identity with mouse and 40% with rat IL-6. IL-6 is phosphorylated at amino acid 81 and it is variably glycosylated by N-linked glycosylation. IL-6 belongs to the IL-6/GCSF/MGF protein family (Rose-John S et al.) whose members share a common use of the gp130 receptor subunit. IL-6 isoforms, with internal deletions, are generated by alternative splicing. The principal cell sources for IL-6 are mononuclear phagocytes, vascular endothelial cells, fibroblasts or other cells.

IL-6 is the ligand for the Interleukin-6 receptor α (IL-6Rα) (Schwantner A et al.) that occurs membrane-bound, but that may also circulate as soluble form generated by alternative splicing or proteolytic cleavage. To induce signaling, IL-6 first forms a complex with the non-signaling IL-6Rα. Subsequent binding to the signal transducing subunit gp130 leads to dimerization of gp130 and finally to the formation of the hexameric signaling complex (Boulanger MJ et al.).

Complexes of IL-6 and soluble IL-6Rα may elicit responses in cells lacking the membrane-bound IL-6Rα but expressing the ubiquitous gp130 coreceptor. This process is known as trans-signaling, it enlarges the spectrum of target cells responding to IL-6 (Mihara M et al.).
产品描述
Method Sandwich ELISA, HRP/TMB, 12×8-well detachable strips Sample type Serum, plasma (EDTA, citrate, heparin), cell culture supernatants, urine Sample volume 100 µl sample / well Assay time 2 h / 1 h / 1h /30 min Sensitivity LOD: 0.28 pg/ml; LLOQ: 0.78 pg/ml (measurable concentrations in serum AND plasma samples!) Standard range 0 – 200 pg/ml (0 / 3.12 / 6.25 / 12.5 / 25/ 50 / 100 / 200) Precision In-between-run (n=3): ≤ 6 % CV Within-run (n=3): ≤ 7 % CV Specificity This assay recognizes recombinant and endogenous (natural) human IL-6 Validation Data See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc
产品特点
保存建议
+4°C
其他
Biomedica Immunoassay(bmgrp)公司成立于1988年, 总部位于奥地利维也纳,主要致力于研究、开发和生产基于ELISA方法用于临床前研究相关标记物检测的工具。迄今为止Biomedica Immunoassay公司在心血管检测及骨代谢检测领域开发了一系列特色ELISA检测试剂盒,Biomedica Immunoassay的生产过程符合GMP/GLP指南,并符合ISO 9001:2015管理体系,有CE认证。Biomedica Immunoassay的产品根据FDA, EMEA和ICH指南进行验证,并具有验证报告。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
在线留言
产品分类
特色产品 MORE
最近促销 MORE
  • 高品质保障 成熟的生产研发技术
  • 高性价比 价宜质优,性价比高
  • 高效省心 从购买到使用,放心无忧
  • 安全运输 完善的保护措施安全运输

微信扫码在线客服

微信咨询

全国免费技术支持

400-6800-868

在线客服