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ELISA试剂盒
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中文名称

游离型可溶的RANKL Elisa试剂盒

英文名字
FREE soluble RANKL HS ELISA Kit
供应商
Biomedica Immunoassays
产品货号
BI-20462
产品报价
¥12x8tests
产品说明书
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产品新闻
背景资料
RANKL, the receptor activator of nuclear factor kappa B ligand, a member of the tumor necrosis factor (TNF) family (http://www.uniprot.org/uniprot/O14788), is the main stimulatory factor for the formation of mature osteoclasts and is essential for their survival. RANKL activates its specific receptor RANK, located on osteoclasts and dendritic cells. The effects are counteracted by Osteoprotegerin (OPG) which acts as an endogenous soluble receptor antagonist (see: OPG ELISA, Cat.No. BI-20403).

The major source of RANKL are osteocytes, former osteoblasts that become embedded within the mineralized bone matrix. RANKL is a ~35 kD type II transmembrane-type protein and is cleaved to release a soluble biologically active product that forms a homotrimer.
产品描述
Method Sandwich ELISA, HRP/TMB, 12×8-well detachable strips Sample type Serum, heparın plasma Sample volume 150 µl / well Assay time 2 h / overnight / 1 h / 30 min Sensitivity 0.01 pmol/l (= 0.2 pg/ml) Standard range 0 – 2 pmol/l (= 0 – 40 pg/ml) Conversion factor 1 pmol/l = 20 pg/ml (MW: 20 kDa; monomer) Specificity Endogenous and recombinant human free soluble RANKL. Precision In-between-run (n=12): ≤ 3 % CV Within-run (n=5): ≤ 5 % CV Cross-reactivity The sequence homology to various primates is >95%. It is likely that the assay can be used for these species.
产品特点
保存建议
+4°C
其他
Biomedica Immunoassay(bmgrp)公司成立于1988年, 总部位于奥地利维也纳,主要致力于研究、开发和生产基于ELISA方法用于临床前研究相关标记物检测的工具。迄今为止Biomedica Immunoassay公司在心血管检测及骨代谢检测领域开发了一系列特色ELISA检测试剂盒,Biomedica Immunoassay的生产过程符合GMP/GLP指南,并符合ISO 9001:2015管理体系,有CE认证。Biomedica Immunoassay的产品根据FDA, EMEA和ICH指南进行验证,并具有验证报告。
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