- 中文名称
清洁帽
- 英文名字
- Clean Cap
- 供应商
- Baseclick
- 产品货号
- BCT-38-S
- 产品报价
- ￥询价/1umol
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 清洁帽(Clean Cap)背景资料,请查看该产品的使用说明书
- 产品描述
- Messenger RNA (mRNA) serves numerous research and clinical purposes. It is produced through the transcription of a DNA template. In vitro transcription (IVT) of mRNA requires efficient capping strategies to generate biologically active and stable mRNA molecules. In mammalian cells, specific molecular structures are added to one end of an mRNA strand to shield it from degradation and ensure proper translation and function. This process is known as capping. Typically, both in vivo and in various lab procedures, the cap is attached after mRNA synthesis, referred to as post-transcriptional or enzymatic capping. This method necessitates additional enzymatic reactions and purification steps, increasing the complexity and duration of the process.TriLink Biotechnologies provides a co-transcriptional capping technology using the CleanCap® molecule, which offers a streamlined, one-pot solution that minimizes the number of steps and results in a cap-1 structure crucial for mRNA function. This, CleanCap®-AG Reagent (BCT-38) is a trinucleotide reagent containing the native cap structure and an A and G at the initial two positions of the mRNA.Conventional capping methods, such as the use of ARCA (anti-reverse cap analogue), sometimes face the problem of capping efficiency and cost. These methods usually result in a cap 0 structure, which can activate pattern recognition receptors and lead to immune responses, reducing the overall efficacy of the mRNA.The CleanCap®-AG molecule binds to the active site of T7 RNA polymerase when it attaches to the DNA template, stabilizing the site. The AG bases of the CleanCap® molecule form base pairs with the DNA template. T7 RNA polymerase can then extend from the CleanCap® molecule by adding new bases, including various modified NTPs, to produce capped RNA products that closely resemble natural mammalian mRNA.CleanCap® reagents advantages: facilitate efficient manufacturing, achieving 95% capping efficiency yielding 3x more product than traditional co-transcriptional methods unlike older techniques reduce production time decrease mRNA immunogenicity enhance stability create the ideal cap-1 structure for superior in vivo performance.PLEASE NOTE: CleanCap® Reagent AG requires an AG initiator.
- 产品形式
- Liquid
- 保存建议
- -20 °C, 避光, 干燥, 无RNA酶
- 其他
- Baseclick于2008成立于2008年德国慕尼黑，BaseClick的核心竞争力源于其专有的铜催化叠氮-炔环加成（CuAAC）点击化学技术。该技术由2001年诺贝尔化学奖得主Barry Sharpless教授发明，BaseClick拥有在核酸领域应用该技术的全球独家许可。相比传统方法，点击化学具有反应迅速、条件温和、产率高、选择性强等显著优势，可实现从小分子寡核苷酸到大基因片段的高效、精准功能化修饰。目前的应用领域包括：核酸标记技术、Edu细胞增殖试验，PCR标记试剂盒，FISH探针，（高度）修饰的寡核苷酸等等，Baseclick跟Thermo Fisher，Roche，Merck，abcam，BD Bioscience等诸多知名品牌建立了合作关系。
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
