- 中文名称
ClickTech DNA FISH 套件 594
- 英文名字
- ClickTech DNA FISH Kit 594
- 供应商
- Baseclick
- 产品货号
- BCK-DNA-FISH-594
- 产品报价
- ￥询价/1kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- ClickTech DNA FISH 套件 594(ClickTech DNA FISH Kit 594)背景资料,请查看该产品的使用说明书
- 产品描述
- Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized.The standard FISH technique, however, often suffers from limitations, namely poor signal to background noise ratio due to unspecific binding of probes to only partially complementary sequences or other cellular and tissue structures. In addition, while even routine in situ detection highly benefits from densely labelled, sensitive probes, the labelling rate of FISH probes generated by traditional methods is typically low.baseclick’s ClickTech DNA FISH Kit overcomes these limitations and provides all reagents required for the generation of highly labelled FISH probes via an easy-to-handle method based on click chemistry. During enzymatic PCR synthesis, unmodified (dNTPs) and alkyne modified- (EdUTP) nucleotides are used by our baseclick Ethynyl Polymerase, producing a clickable PCR product (DNA FISH probe). By using click reagents (incl. in the kit) and a clickable dye (incl. in the kit) of your choice (green 488, yellow 555, orange 594 or red 647) you obtain DNA fluorescent labeled DNA FISH probes.
- 产品特点
- ClickTech DNA FISH 套件 594(ClickTech DNA FISH Kit 594)产品特点,请查看该产品的使用说明书
- 保存建议
- -20 °C
- 其他
- Baseclick于2008成立于2008年德国慕尼黑，BaseClick的核心竞争力源于其专有的铜催化叠氮-炔环加成（CuAAC）点击化学技术。该技术由2001年诺贝尔化学奖得主Barry Sharpless教授发明，BaseClick拥有在核酸领域应用该技术的全球独家许可。相比传统方法，点击化学具有反应迅速、条件温和、产率高、选择性强等显著优势，可实现从小分子寡核苷酸到大基因片段的高效、精准功能化修饰。目前的应用领域包括：核酸标记技术、Edu细胞增殖试验，PCR标记试剂盒，FISH探针，（高度）修饰的寡核苷酸等等，Baseclick跟Thermo Fisher，Roche，Merck，abcam，BD Bioscience等诸多知名品牌建立了合作关系。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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