BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
产品描述
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for WB, IHC, and in situ hybridization.
产品形式
Liquid - clear to very faint yellow/orange
保存建议
Store container at 4° C prior to opening. Protect BCIP/TNBT Membrane Alkaline Phosphatase Substrate from moisture and light. No special shipping conditions or precautions are required.
