- 中文名称
Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay
- 英文名字
- Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- B2851
- 产品报价
- ￥1/50 Slides
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- In the past, most cell-proliferation studies have used radioactive thymidine as an incorporated label to give evidence of DNA replication. Recently, a less hazardous technique has been developed using bromodeoxyuridine (BrdU), a thymidine analog, in lieu of a radioactive reagent. (1) BrdU is incorporated into proliferating cells (S-phase) much in the same way as radioactive thymidine, but is then detected by a monoclonal anti-BrdU antibody, and revealed using a highly sensitive streptavidin- biotin staining system. BrdU has proven useful for proliferative studies of normal and neoplastic tissues both in vivo and in vitro.The BrdU staining system uses a biotinylated monoclonal anti-BrdU, thus eliminating the need for a species-specific secondary antibody. As a result, BrdU labeling can be performed in rats and mice without problems of crossreactivity or spurious staining.Streptavidin-peroxidase is used as a signal generator for the BrdU system. Diaminobenzidine (DAB) in the presence of hydrogen peroxide is used as a chromogen, staining BrdU-incorporated nuclei dark brown. This kit also contains four BrdU control slides for the convenience of the investigator: four unstained positive controls.Kit Components:B2851-01 Trypsin Concentrate 1x3mlB2851-02 Trypsin Diluent 1x12mlB2851-03 Denaturing Solution 1x6ml (Ready-to-use) B2851-04 Blocking Solution 1x6ml(Ready-to-use)B2851-05 BrdU (Biotin) 1x6ml (Ready-to-use) B2851-06 Streptavidin (HRP) 1x6ml (Ready-to-use)B2851-07 Substrate Buffer, 20x 1x2mlB2851-08 DAB, 20x 1x2mlB2851-09 0.6% Hydrogen Peroxide, 20x 1x2mlB2851-10 Hematoxylin 1x6ml (Ready-to-use)B2851-11 Histomount 1x6ml (Ready-to-use) B2851-12 Control Slides 4x Unstained Controls Materials Required but not Provided:-Phosphate Buffered Saline (PBS)-Quenching solution for endogenous peroxidase-Alcohol and xylene-Cover slipsStorage and Stability:Store at 4°C. Stable for at least 6 months.
- 产品特点
- 针对Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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