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细胞分析试剂盒
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中文名称

Brain Derived Neurotrophic Factor (BDNF) BioAssay ELISA Kit

英文名字
Brain Derived Neurotrophic Factor (BDNF) BioAssay ELISA Kit
供应商
USBiological
产品货号
B2700-08
产品报价
¥1/2Plates
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample. Sensitivity: 7.8pg/mlRange of Detection: 7.8-500pg/mlCrossreactivity: No significant crossreactivity with NGF, NT4/5 or NT3 Intra-assay Variation: ± 3.7% (125pg/ml)Inter-assay Variation: ± 8.5% (125pg/ml)Kit Components:There are enough reagents included in this kit for two 96-well immuno-assay plates.B2700-08A: BDNF ELISA Plates, 2x1 plate Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Microtiter plates.B2700-08B: Wash Buffer, 10X, 1x100ml B2700-08C: Standard/Sample Diluent, 1x60ml *B2700-08D: BDNF Standard (Recombinant Human), 2x1 vials (Lyophilized). B2700-08E: Mab (Biotin), 1x25ul B2700-08F: Streptavidin (HRP), 1x50ul B2700-08G: TMB/E Solution, 2x10ml B2700-08H: Stop Solution (HCl), 1x22ml Storage and Stability:Store at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. *B2700-08D: BDNF Standard, Store at 4°C for up to 30 days (liquid).
产品特点
针对Brain Derived Neurotrophic Factor (BDNF) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Brain Derived Neurotrophic Factor (BDNF) BioAssay ELISA Kit产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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