- 中文名称
Beta Defensin 3, Human, BioAssay ELISA Kit (BD3)
- 英文名字
- Beta Defensin 3, Human, BioAssay ELISA Kit (BD3)
- 供应商
- USBiological
- 产品货号
- B0902-06
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Human Beta Defensin 3 (BD-3) ELISA Kit is an in vitro immunoassay for research use in the quantification of BD-3 in cultures of human cells and in appropriately qualified samples from serum, saliva, or other tissue fluids. The kit is suitable for testing a variety of sample types, in accordance with appropriate validation of linearity and recovery.Defensins are cationic, anti-microbial peptides, produced by many cell types (e.g., leukocytes, epithelium, dendrites), and which play prominent roles in the innate immune response of mammals. Two classes have been described in humans, the alpha- and beta-defensins, which range from 3.5-4.5kD and are stabilized by three intramolecular disulfide bonds differing by the ordering of the disulfide bonds in the mature peptides. To date, six alpha-defensins (also referred to as Human Neutrophil Peptides, HNP1-6) and six beta-defensins (HBD1-6) have been investigated. All have demonstrated broad-spectrum in vitro antimicrobial activity against bacteria, fungi and enveloped viruses – activities that are expected to be significant for in vivo protection against pathogens. Researchers have also investigated the ability of defensins to induce the release from tissues of cytokines and chemokines involved in inflammatory and/or adaptive immunity, thereby providing a regulatory role that bridges innate and adaptive immunity. Investigative research has led to the measurement of defensins in serum, saliva, milk, amniotic fluid and lung and cervicovaginal lavage, and culture media of cells and tissue of blood, lung, skin, bowel, muscle, cartilage and kidney, among other sample types.Principle of the Test:The assay is based on the binding of human Beta Defensin 3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to biotin, which then binds to a streptavidin (HRP). After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of BD-3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of BD-3 in samples is calculated from a standard curve of purified recombinant human BD-3 of designated concentration.Range:12.5-400pg/mlKit Components:B0902-06A: Microwell Strips, 1x96 wells (12x8-well strips). Coated with purified anti-human BD3 antibodies. Return unused strips to the pouch with desiccant; re-seal and store refrigerated.B0902-06B: Standard, 400pg/ml, 2x1 vial. Recombinant human BD3. Supplied as a lyophilized powder from buffer, protein stabilizers.B0902-06C: Ab (Biotin), 100X, 1x150ul. Biotinylated anti-human BD3. Supplied as a liquid in buffer, protein, detergents, 0.02% Bronidox. B0902-06D: Streptavidin (HRP), 100X, 1x150ul. Supplied as a liquid in buffer with protein, detergents, 0.02% Bronidox.B0902-06E: Sample Diluent, 10X, 1x10ml.B0902-06F: Biotin, HRP Diluent, 1x22ml. Supplied as a liquid in buffer, protein, anti-microbials.B0902-06G: Wash Solution, 100X, 1x10ml.B0902-06H: TMB Solution, 1x12ml. Chromogenic substrate for HRP containing TMB and peroxide.B0902-06J: Stop Solution (1% H2SO4), 1x12ml. 1% sulfuric acid. Caution: Corrosive!Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Beta Defensin 3, Human, BioAssay ELISA Kit (BD3)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Beta Defensin 3, Human, BioAssay ELISA Kit (BD3)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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